ArticleMolecular endocrinology (Baltimore, Md.)2010
RUNX2 transcription factor regulates gene expression in luteinizing granulosa cells of rat ovaries.
Article in Molecular endocrinology (Baltimore, Md.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 60 citations in OpenAlex.
- Characterization of Ovarian Cell Types and Molecular Signatures in Mauremys reevesii Using Single-Cell Sequencing.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- PGR-mediated transcriptome identifies SOX9 as a critical regulator of progesterone production in human ovulatory follicles.iScience · 2026Article
- Multiomics Analyses Provide New Insight into Genetic Variation of Reproductive Adaptability in Tibetan Sheep.Molecular biology and evolution · 2024Article
- RUN(X) out of blood: emerging RUNX1 functions beyond hematopoiesis and links to Down syndrome.Human genomics · 2023Review
- A single-cell gene expression atlas of human follicular aspirates: Identification of leukocyte subpopulations and their paracrine factors.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Article
- Review
- Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice.Scientific reports · 2020Article
- RUNX3 Promotes the Tumorigenic Phenotype in KGN, a Human Granulosa Cell Tumor-Derived Cell Line.International journal of molecular sciences · 2019Article
- Ovulation: Parallels With Inflammatory Processes.Endocrine reviews · 2019Review
- New insights into the genic and metabolic characteristics of induced pluripotent stem cells from polycystic ovary syndrome women.Stem cell research & therapy · 2018Article
- The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells.International journal of molecular sciences · 2018Article
- Article
- Core Binding Factor β Expression in Ovarian Granulosa Cells Is Essential for Female Fertility.Endocrinology · 2018Article
- Runx3 transcription factor regulates ovarian functions and ovulation in female mice.The Journal of reproduction and development · 2016Article
- Core Binding Factor-β Knockdown Alters Ovarian Gene Expression and Function in the Mouse.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Herp depletion arrests the S phase of the cell cycle and increases estradiol synthesis in mouse granulosa cells.The Journal of reproduction and development · 2016Article
- GnRH regulates trophoblast invasion via RUNX2-mediated MMP2/9 expression.Molecular human reproduction · 2016Article
- Article
- X-linked lymphocyte regulated gene 5c-like (Xlr5c-like) is a novel target of progesterone action in granulosa cells of periovulatory rat ovaries.Molecular and cellular endocrinology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The LH surge promotes terminal differentiation of follicular cells to become luteal cells. RUNX2 has been shown to play an important role in cell differentiation, but the regulation of Runx2 expression and its function in the ovary remain to be determined. The present study examined 1) the expression profile of Runx2 and its partner CBFbeta during the periovulatory period, 2) regulatory mechanisms of Runx2 expression, and 3) its potential function in the ovary. Runx2 expression was induced in periovulatory granulosa cells of human and rodent ovaries. RUNX2 and core binding factor-beta (CBFbeta) proteins in nuclear extracts and RUNX2 binding to a consensus binding sequence increased after human chorionic gonadotropin (hCG) administration. This in vivo up-regulation of Runx2 expression was recapitulated in vitro in preovulatory granulosa cells by stimulation with hCG. The hCG-induced Runx2 expression was reduced by antiprogestin (RU486) and EGF-receptor tyrosine kinase inhibitor (AG1478), indicating the involvement of EGF-signaling and progesterone-mediated pathways. We also found that in the C/EBPbeta knockout mouse ovary, Runx2 expression was reduced, indicating C/EBPbeta-mediated expression. Next, the function of RUNX2 was investigated by suppressing Runx2 expression by small interfering RNA in vitro. Runx2 knockdown resulted in reduced levels of mRNA for Rgc32, Ptgds, Fabp6, Mmp13, and Abcb1a genes. Chromatin immunoprecipitation analysis demonstrated the binding of RUNX2 in the promoter region of these genes, suggesting that these genes are direct downstream targets of RUNX2. Collectively, the present data indicate that the LH surge-induced RUNX2 is involved in various aspects of luteal function by directly regulating the expression of diverse luteal genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.