ArticleJournal of structural biology2010
Automated electron microscopy for evaluating two-dimensional crystallization of membrane proteins.
Article in Journal of structural biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Leginon: New features and applications.Protein science : a publication of the Protein Society · 2021Article
- Software tools for automated transmission electron microscopy.Nature methods · 2019Review
- Structure of the SLC4 transporter Bor1p in an inward-facing conformation.Protein science : a publication of the Protein Society · 2017Article
- Deducing the symmetry of helical assemblies: Applications to membrane proteins.Journal of structural biology · 2016Article
- Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2015Article
- Sparse and incomplete factorial matrices to screen membrane protein 2D crystallization.Journal of structural biology · 2015Article
- Two-Dimensional Crystallization Procedure, from Protein Expression to Sample Preparation.BioMed research international · 2015Review
- Inward-facing conformation of the zinc transporter YiiP revealed by cryoelectron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- High-throughput methods for electron crystallography.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Membrane protein structure determination by electron crystallography.Current opinion in structural biology · 2012Review
- Electron crystallography--the waking beauty of structural biology.Current opinion in structural biology · 2012Review
- Advances in structural and functional analysis of membrane proteins by electron crystallography.Structure (London, England : 1993) · 2011Review
- Specimen preparation for electron diffraction of thin crystals.Micron (Oxford, England : 1993) · 2011Review
- Detection of membrane protein two-dimensional crystals in living cells.Biophysical journal · 2011Article
- An automated pipeline to screen membrane protein 2D crystallization.Journal of structural and functional genomics · 2010Article
- Present and future of membrane protein structure determination by electron crystallography.Advances in protein chemistry and structural biology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Membrane proteins fulfill many important roles in the cell and represent the target for a large number of therapeutic drugs. Although structure determination of membrane proteins has become a major priority, it has proven to be technically challenging. Electron microscopy of two-dimensional (2D) crystals has the advantage of visualizing membrane proteins in their natural lipidic environment, but has been underutilized in recent structural genomics efforts. To improve the general applicability of electron crystallography, high-throughput methods are needed for screening large numbers of conditions for 2D crystallization, thereby increasing the chances of obtaining well ordered crystals and thus achieving atomic resolution. Previous reports describe devices for growing 2D crystals on a 96-well format. The current report describes a system for automated imaging of these screens with an electron microscope. Samples are inserted with a two-part robot: a SCARA robot for loading samples into the microscope holder, and a Cartesian robot for placing the holder into the electron microscope. A standard JEOL 1230 electron microscope was used, though a new tip was designed for the holder and a toggle switch controlling the airlock was rewired to allow robot control. A computer program for controlling the robots was integrated with the Leginon program, which provides a module for automated imaging of individual samples. The resulting images are uploaded into the Sesame laboratory information management system database where they are associated with other data relevant to the crystallization screen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.