ArticleAnnals of biomedical engineering2010
A stretching device for high-resolution live-cell imaging.
Article in Annals of biomedical engineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 76 citations in OpenAlex.
- Biomechanical research using advanced micro-nano devices: In-Vitro cell Characterization focus.Journal of advanced research · 2025Review
- The effects of mechanical stimulation on the morphology and function of spinal ligament cells.BMC musculoskeletal disorders · 2025Article
- StretchView - A Multi-Axial Cell-Stretching Device for Long-Term Automated Videomicroscopy of Living Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- From static to dynamic: The influence of mechanotransduction on skin equivalents analyzed by bioimaging and RNAseq.Materials today. Bio · 2024Article
- Mechanical resilience of the sessile tunicate Botryllus schlosseri.The Journal of experimental biology · 2023Article
- Membrane-Bound Vimentin Filaments Reorganize and Elongate under Strain.Biomacromolecules · 2023Article
- Engineering tools for quantifying and manipulating forces in epithelia.Biophysics reviews · 2023Review
- The CaT stretcher: An open-source system for delivering uniaxial strain to cells and tissues (CaT).Frontiers in bioengineering and biotechnology · 2022Article
- An Easy-to-Fabricate Cell Stretcher Reveals Density-Dependent Mechanical Regulation of Collective Cell Movements in Epithelia.Cellular and molecular bioengineering · 2021Article
- Development of an Aged Full-Thickness Skin Model Using Flexible Skin-on-a-Chip Subjected to Mechanical Stimulus Reflecting the Circadian Rhythm.International journal of molecular sciences · 2021Article
- A Fully Integrated Arduino-Based System for the Application of Stretching Stimuli to Living Cells and Their Time-Lapse Observation: A Do-It-Yourself Biology Approach.Annals of biomedical engineering · 2021Article
- Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction.Biology open · 2021Article
- Studying the Mechanobiology of Aortic Endothelial Cells Under Cyclic Stretch Using a Modular 3D Printed System.Frontiers in bioengineering and biotechnology · 2021Article
- TENSCell: Imaging of Stretch-Activated Cells Reveals Divergent Nuclear Behavior and Tension.Biophysical journal · 2020Article
- Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes.Biomechanics and modeling in mechanobiology · 2020Article
- Review
- Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress.Scientific reports · 2018Article
- Biochemical analysis of force-sensitive responses using a large-scale cell stretch device.Cell adhesion & migration · 2017Article
- An Electromagnetically Actuated Double-Sided Cell-Stretching Device for Mechanobiology Research.Micromachines · 2017Article
- Computationally Informed Design of a Multi-Axial Actuated Microfluidic Chip Device.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Several custom-built and commercially available devices are available to investigate cellular responses to substrate strain. However, analysis of structural dynamics by microscopy in living cells during stretch is not readily feasible. We describe a novel stretch device optimized for high-resolution live-cell imaging. The unit assembles onto standard inverted microscopes and applies constant magnitude or cyclic stretch at physiological magnitudes to cultured cells on elastic membranes. Interchangeable modular indenters enable delivery of equibiaxial and uniaxial stretch profiles. Strain analysis performed by tracking fluorescent microspheres adhered onto the substrate demonstrated reproducible application of stretch profiles. In endothelial cells transiently expressing enhanced green fluorescent protein (EGFP)-vimentin and paxillin-DsRed2 and subjected to constant magnitude equibiaxial stretch, the two-dimensional strain tensor demonstrated efficient transmission through the extracellular matrix and focal adhesions. Decreased transmission to the intermediate filament network was measured, and a heterogeneous spatial distribution of maximum stretch magnitude revealed discrete sites of strain focusing. Spatial correlation of vimentin and paxillin displacement vectors provided an estimate of the extent of mechanical coupling between the structures. Interestingly, switching the spatial profile of substrate strain reveals that actin-mediated edge ruffling is not desensitized to repeated mechanostimulation. These initial observations show that the stretch device is compatible with live-cell microscopy and is a novel tool for measuring dynamic structural remodeling under mechanical strain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.