ArticleJournal of fluorescence2010
Origin of fluorescence lifetimes in human serum albumin. Studies on native and denatured protein.
Article in Journal of fluorescence, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
- Organic-solvent mediated self-assembly of protoporphyrin IX with human serum albumin.International journal of biological macromolecules · 2025Article
- Revisiting and Updating the Interaction between Human Serum Albumin and the Non-Steroidal Anti-Inflammatory Drugs Ketoprofen and Ketorolac.Molecules (Basel, Switzerland) · 2024Article
- Time-Resolved Fluorescence Spectroscopy of Blood, Plasma and Albumin as a Potential Diagnostic Tool for Acute Inflammation in COVID-19 Pneumonia Patients.International journal of molecular sciences · 2023Article
- 3-Ferrocenyl-estra-1,3,5 (10)-triene-17-one: Synthesis, Crystal Structure, Hirshfeld Surface Analysis, DFT Studies, and Its Binding to Human Serum Albumin Studied through Fluorescence Quenching and In Silico Docking Studies.Molecules (Basel, Switzerland) · 2023Article
- Tuning anticancer properties and DNA-binding of Pt(ii) complexesRSC advances · 2023Article
- Biophysical Characterization of the Interaction between a Transport Human Plasma Protein and the 5,10,15,20-Tetra(pyridine-4-yl)porphyrin.Molecules (Basel, Switzerland) · 2022Article
- A study of the oxidative processes in human plasma by time-resolved fluorescence spectroscopy.Scientific reports · 2022Article
- Alteration of Anticancer and Protein-Binding Properties of Gold(I) Alkynyl by Phenolic Schiff Bases Moieties.Pharmaceutics · 2021Article
- Interaction with Human Serum Proteins Reveals Biocompatibility of Phosphocholine-Functionalized SPIONs and Formation of Albumin-Decorated Nanoparticles.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Molecular Interaction of Amino Acid-Based Gemini Surfactant with Human Serum Albumin: Tensiometric, Spectroscopic, and Molecular Docking Study.ACS omega · 2019Article
- Novel combinations of experimental and computational analysis tested on the binding of metalloprotoporphyrins to albumin.International journal of biological macromolecules · 2019Article
- Biophysical characterization of the interaction of human albumin with an anionic porphyrin.Biochemistry and biophysics reports · 2016Article
- Molecular docking, a tool to determine interaction of CuO and TiOBiochemistry and biophysics reports · 2016Article
- Origin of tryptophan fluorescence lifetimes. Part 2: fluorescence lifetimes origin of tryptophan in proteins.Journal of fluorescence · 2014Article
- Interaction of the iron(II) cage complexes with proteins: protein fluorescence quenching study.Journal of fluorescence · 2013Article
- Interaction of human serum albumin with novel 3,9-disubstituted perylenes.The protein journal · 2013Article
- Sub-structures formed in the excited state are responsible for tryptophan residues fluorescence in β-lactoglobulin.Journal of fluorescence · 2011Article
- Relation between proteins tertiary structure, tryptophan fluorescence lifetimes and tryptophan S(o)→(1)L(b) and S(o)→(1)L(a) transitions. Studies on α1-acid glycoprotein and β-lactoglobulin.Journal of fluorescence · 2011Article
- Effect of 1-aminoanthracene (1-AMA) binding on the structure of three lipocalin proteins, the dimeric β lactoglobulin, the dimeric odorant binding protein and the monomeric α1-acid glycoprotein. Fluorescence spectra and lifetimes studies.Journal of fluorescence · 2010Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human serum albumin consists of a single polypeptide of 585 amino acid residues with 1 Trp residue. In the present work, we measured fluorescence lifetimes of the protein in both native and denatured states. The results indicate that Trp emission occurs with three lifetimes in both states. Lifetimes values and contribution to the global emission decay differ between the two states. Data are interpreted as the results of an emission occurring from three substructures of the tryptophan formed in the excited state. Two of these substructures are already present for the tryptophan free in solution. The third lifetime is the result of the interaction between the tryptophan residue and surrounding microenvironment. The populations of these substructures characterized by the pre-exponential parameters of the fluorescence lifetimes are dependent on the fluorophore microenvironment and on the global protein structure.
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Registered trials
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