ArticlePlant cell reports2010
Upregulation of phytosterol and triterpene biosynthesis in Centella asiatica hairy roots overexpressed ginseng farnesyl diphosphate synthase.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 117 citations in OpenAlex.
- Review
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- The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.International journal of molecular sciences · 2024Review
- Telomere-to-telomere reference genome forHorticulture research · 2024Article
- Enhancing Centelloside Production inPlants (Basel, Switzerland) · 2023Article
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- Metabolic Modifications in Terpenoid and Steroid Pathways Triggered by Methyl Jasmonate inPlants (Basel, Switzerland) · 2022Article
- Enhancement of Phytosterol and Triterpenoid Production in Plant Hairy Root Cultures-Simultaneous Stimulation or Competition?Plants (Basel, Switzerland) · 2021Review
- Unraveling dynamic metabolomes underlying different maturation stages of berries harvested fromJournal of ginseng research · 2020Article
- Effects of methyl jasmonate on the growth and triterpenoid production of diploid and tetraploid Centella asiatica (L.) Urb. hairy root cultures.Scientific reports · 2019Article
- Elicitation with methyl jasmonate combined with cultivation in the Plantform™ temporary immersion bioreactor highly increases the accumulation of selected centellosides and phenolics inEngineering in life sciences · 2019Article
- Identification of key genes involved in the biosynthesis of triterpenic acids in the mint family.Scientific reports · 2019Article
- Plant triterpenoid saponins: biosynthesis, in vitro production, and pharmacological relevance.Protoplasma · 2019Review
- Effects of topical applications of porcine acellular urinary bladder matrix and Centella asiatica extract on oral wound healing in a rat model.Clinical oral investigations · 2019Article
- Elicitation Enhanced the Yield of Glycyrrhizin and Antioxidant Activities in Hairy Root Cultures ofJournal of plant growth regulation · 2019Article
- The Effects of Environmental Factors on Ginsenoside Biosynthetic Enzyme Gene Expression and Saponin Abundance.Molecules (Basel, Switzerland) · 2018Article
- Progress on the Studies of the Key Enzymes of Ginsenoside Biosynthesis.Molecules (Basel, Switzerland) · 2018Review
- Strengthening Triterpene Saponins Biosynthesis by Over-Expression of Farnesyl Pyrophosphate Synthase Gene and RNA Interference of Cycloartenol Synthase Gene in Panax notoginseng Cells.Molecules (Basel, Switzerland) · 2017Article
- Genetic Transformation System for Woody PlantFrontiers in plant science · 2017Article
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Farnesyl diphosphate synthase (FPS) plays an essential role in organ development in plants. However, FPS has not previously been identified as a key regulatory enzyme in triterpene biosynthesis. To elucidate the functions of FPS in triterpene biosynthesis, C. asiatica was transformed with a construct harboring Panax ginseng FPS (PgFPS)-encoding cDNA coupled to the cauliflower mosaic virus 35S promoter. Higher levels of CaDDS (C. asiatica dammarenediol synthase) and CaCYS (C. asiatica cycloartenol synthase) mRNA were detected in all hairy root lines overexpressing when compared with the controls. However, no differences were detected in any expression of the CaSQS (C. asiatica squalene synthase) gene. In particular, the upregulation of CaDDS transcripts suggests that FPS may result in alterations in triterpene biosynthesis capacity. Squalene contents in the T17, T24, and T27 lines were increased to 1.1-, 1.3- and 1.5-fold those in the controls, respectively. The total sterol contents in the T24 line were approximately three times higher than those of the controls. Therefore, these results indicated that FPS performs a regulatory function in phytosterol biosynthesis. To evaluate the contribution of FPS to triterpene biosynthesis, we applied methyl jasmonate as an elicitor of hairy roots expressing PgFPS. The results of HPLC analysis revealed that the content of madecassoside and asiaticoside in the T24 line was transiently increased by 1.15-fold after 14 days of MJ treatment. This result may indicate that FPS performs a role not only in phytosterol regulation, but also in triterpene biosynthesis.
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