ArticlePloS one2010
An atypical riboflavin pathway is essential for Brucella abortus virulence.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- Metabolic map of Brucellosis: From acute and chronic phase adaptation to targeted nanodrug design.Materials today. Bio · 2026Review
- INM004: Polyclonal Neutralizing Antibodies Against Shiga Toxin as a Treatment for Hemolytic Uremic Syndrome.Toxins · 2025Review
- Article
- Improvement of Cellulomonas fimi endoglucanase CenA by multienzymatic display on a decameric structural scaffold.Applied microbiology and biotechnology · 2023Article
- Comparative Genomic Analysis of Fusobacterium necrophorum Provides Insights into Conserved Virulence Genes.Microbiology spectrum · 2022Article
- RibU is an essential determinant ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Review
- Article
- Evasion of MAIT cell recognition by the AfricanProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Molecular Elucidation of Riboflavin Production and Regulation in Candida albicans, toward a Novel Antifungal Drug Target.mSphere · 2020Article
- Article
- Asymmetric bifunctional protein nanoparticles through redesign of self-assembly.Nanoscale advances · 2019Article
- Transcriptomics reveals a cross-modulatory effect between riboflavin and iron and outlines responses to riboflavin biosynthesis and uptake in Vibrio cholerae.Scientific reports · 2018Article
- Platelets PromoteFrontiers in immunology · 2018Article
- The superfamily keeps growing: Identification in trypanosomatids of RibJ, the first riboflavin transporter family in protists.PLoS neglected tropical diseases · 2017Article
- Contribution of riboflavin supply pathways toGut pathogens · 2017Article
- Differential regulation of riboflavin supply genes inGut pathogens · 2017Article
- Vitamin B2 as a virulence factor in Pseudogymnoascus destructans skin infection.Scientific reports · 2016Article
- MAIT, MR1, microbes and riboflavin: a paradigm for the co-evolution of invariant TCRs and restricting MHCI-like molecules?Immunogenetics · 2016Review
- Targeting Innate-Like T Cells in Tuberculosis.Frontiers in immunology · 2016Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Brucellosis is a worldwide zoonosis that affects livestock and humans and is caused by closely related Brucella spp., which are adapted to intracellular life within cells of a large variety of mammals. Brucella can be considered a furtive pathogen that infects professional and non-professional phagocytes. In these cells Brucella survives in a replicative niche, which is characterized for having a very low oxygen tension and being deprived from nutrients such as amino acids and vitamins. Among these vitamins, we have focused on riboflavin (vitamin B2). Flavin metabolism has been barely implicated in bacterial virulence. We have recently described that Brucella and other Rhizobiales bear an atypical riboflavin metabolic pathway. In the present work we analyze the role of the flavin metabolism on Brucella virulence. Mutants on the two lumazine synthases (LS) isoenzymes RibH1 and RibH2 and a double RibH mutant were generated. These mutants and different complemented strains were tested for viability and virulence in cells and in mice. In this fashion we have established that at least one LS must be present for B. abortus survival and that RibH2 and not RibH1 is essential for intracellular survival due to its LS activity in vivo. In summary, we show that riboflavin biosynthesis is essential for Brucella survival inside cells or in mice. These results highlight the potential use of flavin biosynthetic pathway enzymes as targets for the chemotherapy of brucellosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.