ArticlePLoS pathogens2010
Vaccinia protein F12 has structural similarity to kinesin light chain and contains a motor binding motif required for virion export.
Article in PLoS pathogens, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 48 citations in OpenAlex.
- Lumpy Skin Disease Virus Pathogenesis: Viral Protein Functions and Comparative Insights from Vaccinia Virus.Animals : an open access journal from MDPI · 2025Review
- Temporal expression classes and functions of vaccinia virus and mpox (monkeypox) virus genes.mBio · 2025Review
- MPXV: Update on Morphological and Morphogenesis Aspects Through Transmission and Scanning Electron Microscopies and 3D Reconstruction.Journal of medical virology · 2025Article
- TRIM Proteins and Antiviral Microtubule Reorganization: A Novel Component in Innate Immune Responses?Viruses · 2024Review
- Review
- The crystal structure of vaccinia virus protein E2 and perspectives on the prediction of novel viral protein folds.The Journal of general virology · 2022Article
- Potential link of single nucleotide polymorphisms to virulence of vaccine-associated field strains of lumpy skin disease virus in South Africa.Transboundary and emerging diseases · 2020Article
- LMTK2 binds to kinesin light chains to mediate anterograde axonal transport of cdk5/p35 and LMTK2 levels are reduced in Alzheimer's disease brains.Acta neuropathologica communications · 2019Article
- Wolbachia and host germline components compete for kinesin-mediated transport to the posterior pole of the Drosophila oocyte.PLoS pathogens · 2018Article
- Tagging of the vaccinia virus protein F13 with mCherry causes aberrant virion morphogenesis.The Journal of general virology · 2017Article
- Vaccinia virus proteins A36 and F12/E2 show strong preferences for different kinesin light chain isoforms.Traffic (Copenhagen, Denmark) · 2017Article
- Vaccinia virus egress mediated by virus protein A36 is reliant on the F12 protein.The Journal of general virology · 2017Article
- Trichoplusia ni Kinesin-1 Associates with Autographa californica Multiple Nucleopolyhedrovirus Nucleocapsid Proteins and Is Required for Production of Budded Virus.Journal of virology · 2016Article
- Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex.PLoS pathogens · 2015Article
- Chordopoxvirus protein F12 implicated in enveloped virion morphogenesis is an inactivated DNA polymerase.Biology direct · 2014Article
- Article
- Electron microscopy: essentials for viral structure, morphogenesis and rapid diagnosis.Science China. Life sciences · 2013Review
- Structural basis for kinesin-1:cargo recognition.Science (New York, N.Y.) · 2013Article
- A36-dependent actin filament nucleation promotes release of vaccinia virus.PLoS pathogens · 2013Article
- Modulation of the myxoma virus plaque phenotype by vaccinia virus protein F11.Journal of virology · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Vaccinia virus (VACV) uses microtubules for export of virions to the cell surface and this process requires the viral protein F12. Here we show that F12 has structural similarity to kinesin light chain (KLC), a subunit of the kinesin-1 motor that binds cargo. F12 and KLC share similar size, pI, hydropathy and cargo-binding tetratricopeptide repeats (TPRs). Moreover, molecular modeling of F12 TPRs upon the crystal structure of KLC2 TPRs showed a striking conservation of structure. We also identified multiple TPRs in VACV proteins E2 and A36. Data presented demonstrate that F12 is critical for recruitment of kinesin-1 to virions and that a conserved tryptophan and aspartic acid (WD) motif, which is conserved in the kinesin-1-binding sequence (KBS) of the neuronal protein calsyntenin/alcadein and several other cellular kinesin-1 binding proteins, is essential for kinesin-1 recruitment and virion transport. In contrast, mutation of WD motifs in protein A36 revealed they were not required for kinesin-1 recruitment or IEV transport. This report of a viral KLC-like protein containing a KBS that is conserved in several cellular proteins advances our understanding of how VACV recruits the kinesin motor to virions, and exemplifies how viruses use molecular mimicry of cellular components to their advantage.
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