ArticleAging2009
Oxidative modification and aggregation of creatine kinase from aged mouse skeletal muscle.
Article in Aging, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Mitochondrial oxidative stress in skeletal muscle alters metabolic signaling responses to acute muscle contraction.Free radical biology & medicine · 2026Article
- Decreased methylglyoxal-mediated protein glycation in the healthy aging mouse model of ectopic expression of UCP1 in skeletal muscle.Redox biology · 2023Article
- Fiber-Type Shifting in Sarcopenia of Old Age: Proteomic Profiling of the Contractile Apparatus of Skeletal Muscles.International journal of molecular sciences · 2023Review
- Oxidative damage in metal fragment-embedded Sprague-Dawley rat gastrocnemius muscle.Current research in toxicology · 2022Article
- Effect of statin treatment in obese selenium-supplemented mice lacking selenocysteine lyase.Molecular and cellular endocrinology · 2021Article
- Acetylation of muscle creatine kinase negatively impacts high-energy phosphotransfer in heart failure.JCI insight · 2021Article
- Proteomic Analysis of Uterine Tissues During Peri-Implantation Period in Mice with Experimentally Induced Adenomyosis that Treated with anti-Ngf: Implications for Cell-Cell Adhesion and Metabolic Processes.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Exercise intolerance and rapid skeletal muscle energetic decline in human age-associated frailty.JCI insight · 2020Article
- High-resolution genome-wide expression analysis of single myofibers using SMART-Seq.The Journal of biological chemistry · 2019Article
- Overweight in the Elderly Induces a Switch in Energy Metabolism that Undermines Muscle Integrity.Aging and disease · 2019Article
- Article
- Mitochondrial Superoxide Increases Age-Associated Susceptibility of Human Dermal Fibroblasts to Radiation and Chemotherapy.Cancer research · 2017Article
- Changes in the mitochondrial function and in the efficiency of energy transfer pathways during cardiomyocyte aging.Molecular and cellular biochemistry · 2017Article
- Liver morphometrics and metabolic blood profile across divergent phenotypes for feed efficiency in the bovine.Acta veterinaria Scandinavica · 2017Article
- Absence of Dystrophin Disrupts Skeletal Muscle Signaling: Roles of Ca2+, Reactive Oxygen Species, and Nitric Oxide in the Development of Muscular Dystrophy.Physiological reviews · 2016Review
- The Measurement of Reversible Redox Dependent Post-translational Modifications and Their Regulation of Mitochondrial and Skeletal Muscle Function.Frontiers in physiology · 2015Review
- Proteomics of muscle chronological ageing in post-menopausal women.BMC genomics · 2014Article
- Skeletal muscle ATP kinetics are impaired in frail mice.Age (Dordrecht, Netherlands) · 2014Article
- Oxidative stress in aging: advances in proteomic approaches.Oxidative medicine and cellular longevity · 2014Review
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Creatine kinase catalyzes the reversible transfer of the gamma phosphate from ATP to creatine forming the high energy compound creatine phosphate. Muscle creatine kinase (CKm) activity maintains energetic homeostasis as variations in energy requirements dictate that ATP be readily available. Recent studies suggest that CKm activity is altered during aging. Proteomic analyses have shown that CKm is 3-nitrotyrosine (3-NT) modified and carbonylated in aged rodent skeletal muscle. However, it remains unknown if these modifications affect its structure and activity. To address this we characterized oxidatively modified CKm from the quadriceps of young, middle-aged, and aged mice. Our data indicate that 3-NT modified and carbonylated CKm are found predominantly in aged muscle and that it exists in high molecular weight oligomers and insoluble protein aggregates. CKm from middle-aged and aged mouse quadriceps also exhibits structural instability that may account for its reduction in function. These structural and functional changes correlate with the differential protein modifications. Interestingly, the majority of the age-related changes in enzyme activity and protein stability occurred by middle age. Our studies indicate that the age-associated oxidative and nitrative modification of CKm results in a decrease in its activity and may cause structural changes that promote oligomerization and aggregation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.