ArticleAmerican journal of respiratory and critical care medicine2010
Induction of tissue factor by urokinase in lung epithelial cells and in the lungs.
Article in American journal of respiratory and critical care medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Culture with apically applied healthy or disease sputum alters the airway surface liquid proteome and ion transport across human bronchial epithelial cells.American journal of physiology. Cell physiology · 2021Article
- Lung Epithelial Cell Transcriptional Regulation as a Factor in COVID-19-associated Coagulopathies.American journal of respiratory cell and molecular biology · 2021Article
- Unique transcriptional changes in coagulation cascade genes in SARS-CoV-2-infected lung epithelial cells: A potential factor in COVID-19 coagulopathies.bioRxiv : the preprint server for biology · 2020Article
- Infection-induced thrombin production: a potential novel mechanism for preterm premature rupture of membranes (PPROM).American journal of obstetrics and gynecology · 2018Article
- The role of mononuclear cell tissue factor and inflammatory cytokines in patients with chronic thromboembolic pulmonary hypertension.Journal of thrombosis and thrombolysis · 2016Article
- Involvement of urokinase in cigarette smoke extract-induced epithelial-mesenchymal transition in human small airway epithelial cells.Laboratory investigation; a journal of technical methods and pathology · 2015Article
- The inflammatory actions of coagulant and fibrinolytic proteases in disease.Mediators of inflammation · 2015Review
- Plasminogen activator inhibitor-1 in cigarette smoke exposure and influenza A virus infection-induced lung injury.PloS one · 2015Article
- Expression of tissue factor in rabbit pulmonary artery in an acute pulmonary embolism model.World journal of emergency medicine · 2014Article
- Tissue factor signals airway epithelial basal cell survival via coagulation and protease-activated receptor isoforms 1 and 2.American journal of respiratory cell and molecular biology · 2013Article
- Tissue factor-bearing exosome secretion from human mechanically stimulated bronchial epithelial cells in vitro and in vivo.The Journal of allergy and clinical immunology · 2012Article
- Regulation of airway and alveolar epithelial cell apoptosis by p53-Induced plasminogen activator inhibitor-1 during cigarette smoke exposure injury.American journal of respiratory cell and molecular biology · 2012Article
- Characterization of coagulation factor synthesis in nine human primary cell types.Scientific reports · 2012Article
- Update in acute lung injury and critical care 2010.American journal of respiratory and critical care medicine · 2011Review
- Tissue factor, blood coagulation, and beyond: an overview.International journal of inflammation · 2011Article
- Induction of tissue factor by urokinase in lung epithelial cells and in the lungs.American journal of respiratory and critical care medicine · 2010Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
Abstract
rationaleUrokinase-type plasminogen activator (uPA) regulates extracellular proteolysis in lung injury and repair. Although alveolar expression of uPA increases, procoagulant activity predominates.
objectivesThis study was designed to investigate whether uPA alters the expression of tissue factor (TF), the major initiator of the coagulation cascade, in lung epithelial cells (ECs).
methodsBronchial, primary airway ECs and C57B6 wild-type, uPA-deficient (uPA(-/-)) mice were exposed to phosphate-buffered saline, uPA, or LPS. Immunohistochemistry, protein, cellular, and molecular techniques were used to assess TF expression and activity. MEASUREMENTS AND MAIN
resultsuPA enhanced TF mRNA and protein expression, and TF-dependent coagulation in lung ECs. uPA-induced expression of TF involves both increased synthesis and enhanced stabilization of TF mRNA. uPA catalytic activity had little effect on induction of TF. By contrast, deletion of the uPA receptor binding growth factor domain from uPA markedly attenuated the induction of TF, suggesting that uPA receptor binding is sufficient for TF induction. Lung tissues of uPA-deficient mice expressed less TF protein and mRNA compared with wild-type mice. In addition, intratracheal instillation of mouse uPA increased TF mRNA and protein expression and accelerated coagulation in lung tissues. uPA(-/-) mice exposed to LPS failed to induce TF.
conclusionsuPA increased TF expression and TF-dependent coagulation in the lungs of mice. We hypothesize that uPA-mediated induction of TF occurs in lung ECs to promote increased fibrin deposition in the airways during acute lung injury.
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