ArticleProceedings of the National Academy of Sciences of the United States of America2010
Reduced voltage sensitivity in a K+-channel voltage sensor by electric field remodeling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- HInternational journal of molecular sciences · 2025Review
- Effect of a sensing charge mutation on the deactivation of KV7.2 channels.The Journal of general physiology · 2024Article
- Sweetening K-channels: what sugar taught us about permeation and gating.Frontiers in molecular biosciences · 2023Review
- Binding of κ-Conotoxin-PVIIA to Open and Closed Shaker K-Channels Are Differentially Affected by the Ionic Strength.Marine drugs · 2020Article
- A novel theoretical framework reveals more than one voltage-sensing pathway in the lateral membrane of outer hair cells.The Journal of general physiology · 2020Article
- Novel TRPV1 Channel Agonists With Faster and More Potent Analgesic Properties Than Capsaicin.Frontiers in pharmacology · 2020Article
- Article
- Linkage analysis reveals allosteric coupling in Kir2.1 channels.The Journal of general physiology · 2018Article
- The Role of Proton Transport in Gating Current in a Voltage Gated Ion Channel, as Shown by Quantum Calculations.Sensors (Basel, Switzerland) · 2018Article
- Gating-induced large aqueous volumetric remodeling and aspartate tolerance in the voltage sensor domain of Shaker KProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Pore size matters for potassium channel conductance.The Journal of general physiology · 2016Review
- Functional diversity of potassium channel voltage-sensing domains.Channels (Austin, Tex.) · 2016Review
- Voltage-dependent gating and gating charge measurements in the Kv1.2 potassium channel.The Journal of general physiology · 2015Article
- Voltage sensor of ion channels and enzymes.Biophysical reviews · 2012Review
- Voltage gated ion channel function: gating, conduction, and the role of water and protons.International journal of molecular sciences · 2012Review
- Interaction of diverse voltage sensor homologs with lipid bilayers revealed by self-assembly simulations.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Propagation of the nerve impulse relies on the extreme voltage sensitivity of Na(+) and K(+) channels. The transmembrane movement of four arginine residues, located at the fourth transmembrane segment (S4), in each of their four voltage-sensing domains is mostly responsible for the translocation of 12 to 13 e(o) across the transmembrane electric field. Inserting additional positively charged residues between the voltage-sensing arginines in S4 would, in principle, increase voltage sensitivity. Here we show that either positively or negatively charged residues added between the two most external sensing arginines of S4 decreased voltage sensitivity of a Shaker voltage-gated K(+)-channel by up to approximately 50%. The replacement of Val363 with a charged residue displaced inwardly the external boundaries of the electric field by at least 6 A, leaving the most external arginine of S4 constitutively exposed to the extracellular space and permanently excluded from the electric field. Both the physical trajectory of S4 and its electromechanical coupling to open the pore gate seemed unchanged. We propose that the separation between the first two sensing charges at resting is comparable to the thickness of the low dielectric transmembrane barrier they must cross. Thus, at most a single sensing arginine side chain could be found within the field. The conserved hydrophobic nature of the residues located between the voltage-sensing arginines in S4 may shape the electric field geometry for optimal voltage sensitivity in voltage-gated ion channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.