ArticleProceedings of the National Academy of Sciences of the United States of America2010
A single mutation in the first transmembrane domain of yeast COX2 enables its allotopic expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 47 citations in OpenAlex.
- TargetMITO: a rule-based model for generating functional synthetic mitochondrial targeting sequences in yeast.Nucleic acids research · 2026Article
- The IQ-compete assay for measuring mitochondrial protein import efficiencies in living yeast cells.FEBS letters · 2026Article
- A protein-specific priority code in presequences determines the efficiency of mitochondrial protein import.PLoS biology · 2025Article
- Article
- A high copy suppressor screen identifies factors enhancing the allotopic production of subunit II of cytochrome c oxidase.G3 (Bethesda, Md.) · 2025Article
- Identification of factors limiting the allotopic production of the Cox2 subunit of yeast cytochrome c oxidase.Genetics · 2024Article
- Mitochondrial genomes revisited: why do different lineages retain different genes?BMC biology · 2024Review
- Engineering artificial photosynthetic life-forms through endosymbiosis.Nature communications · 2022Article
- The Mitochondrial Genome in Aging and Disease and the Future of Mitochondrial Therapeutics.Biomedicines · 2022Review
- Fates of Sec, Tat, and YidC Translocases in Mitochondria and Other Eukaryotic Compartments.Molecular biology and evolution · 2021Review
- Allotopic Expression of ATP6 in Mouse as a Transgenic Model of Mitochondrial Disease.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Allotopic expression of mitochondrial genes: Basic strategy and progress.Genes & diseases · 2020Review
- Codon optimization is an essential parameter for the efficient allotopic expression of mtDNA genes.Redox biology · 2020Article
- Mitochondrial cysteinyl-tRNA synthetase is expressed via alternative transcriptional initiation regulated by energy metabolism in yeast cells.The Journal of biological chemistry · 2019Article
- Toward a Synthetic Yeast Endosymbiont with a Minimal Genome.Journal of the American Chemical Society · 2019Article
- Key within-membrane residues and precursor dosage impact the allotopic expression of yeast subunit II of cytochromeMolecular biology of the cell · 2019Article
- Engineering yeast endosymbionts as a step toward the evolution of mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mitochondrial versus nuclear gene expression and membrane protein assembly: the case of subunit 2 of yeast cytochromeMolecular biology of the cell · 2018Article
- Intercompartmental Piecewise Gene Transfer.Genes · 2017Review
- Aep3p-dependent translation of yeast mitochondrialMolecular biology of the cell · 2017Article
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4 authors at 2 institutions in 1 country.
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Abstract
During the course of evolution, a massive reduction of the mitochondrial genome content occurred that was associated with transfer of a large number of genes to the nucleus. To further characterize factors that control the mitochondrial gene transfer/retention process, we have investigated the barriers to transfer of yeast COX2, a mitochondrial gene coding for a subunit of cytochrome c oxidase complex. Nuclear-recoded Saccharomyces cerevisiae COX2 fused at the amino terminus to various alternative mitochondrial targeting sequences (MTS) fails to complement the growth defect of a yeast strain with an inactivated mitochondrial COX2 gene, even though it is expressed in cells. Through random mutagenesis of one such hybrid MTS-COX2, we identified a single mutation in the first Cox2 transmembrane domain (W56 --> R) that (i) results in the cellular expression of a Cox2 variant with a molecular mass indicative of MTS cleavage, which (ii) supports growth of a cox2 mutant on a nonfermentable carbon source, and that (iii) partially restores cytochrome c oxidase-specific respiration by the mutant mitochondria. COX2(W56R) can be allotopically expressed with an MTS derived from S. cerevisiae OXA1 or Neurospora crassa SU9, both coding for hydrophobic mitochondrial proteins, but not with an MTS derived from the hydrophilic protein Cox4. In contrast to some other previously transferred genes, allotopic COX2 expression is not enabled or enhanced by a 3'-UTR that localizes mRNA translation to the mitochondria, such as yeast ATP2(3)('-UTR). Application of in vitro evolution strategies to other mitochondrial genes might ultimately lead to yeast entirely lacking the mitochondrial genome, but still possessing functional respiratory capacity.
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