ArticleAntimicrobial agents and chemotherapy2010
Use of a high-resolution melt assay to characterize codon 54 of the cyp51A gene of Aspergillus fumigatus on a Rotor-Gene 6000 instrument.
Article in Antimicrobial agents and chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Detecting Azole-Antifungal Resistance inJournal of fungi (Basel, Switzerland) · 2020Review
- Development and Validation of a High-Resolution Melting Assay To Detect Azole Resistance in Aspergillus fumigatus.Antimicrobial agents and chemotherapy · 2017Article
- Detection ofFrontiers in microbiology · 2016Article
- Rapid detection and identification of Aspergillus from lower respiratory tract specimens by use of a combined probe-high-resolution melting analysis.Journal of clinical microbiology · 2012Article
- Antifungal drug resistance: do molecular methods provide a way forward?Current opinion in infectious diseases · 2009Review
- An update on current and novel molecular diagnostics for the diagnosis of invasive fungal infections.Expert review of molecular diagnosticsReview
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high-resolution melt (HRM) assay using a Rotor-Gene 6000 instrument was developed to characterize the codon for glycine 54 in the cyp51A genes from 13 reference isolates and 12 clinical isolates of Aspergillus fumigatus. Mutations in this codon confer reduced susceptibility to itraconazole and posaconazole. The assay is simple to perform, and a result of "wild type" or "mutant" is available after approximately 1 h following DNA extraction using commercially available reagents and conventional primers.
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