ArticleThe Journal of cell biology2010
Mitotic chromosomes are constrained by topoisomerase II-sensitive DNA entanglements.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 64 citations in OpenAlex.
- Motorized chromosome models of mitotic chromosome folding.Nature communications · 2025Article
- Functional interplay between condensin I and topoisomerase Iiα in single-molecule DNA compaction.Nature communications · 2025Article
- Mitotic genome folding.The Journal of cell biology · 2025Review
- Isolation and manipulation of meiotic spindles from mouse oocytes reveals migration regulated by pulling force during asymmetric division.bioRxiv : the preprint server for biology · 2025Article
- Supra-second tracking and live-cell karyotyping reveal principles of mitotic chromosome dynamics.Nature cell biology · 2025Article
- Review
- Mitotic chromosomes are self-entangled and disentangle through a topoisomerase-II-dependent two-stage exit from mitosis.Molecular cell · 2024Article
- The material properties of mitotic chromosomes.Current opinion in structural biology · 2023Review
- Stochastic motion and transcriptional dynamics of pairs of distal DNA loci on a compacted chromosome.Science (New York, N.Y.) · 2023Article
- Stochastic motion and transcriptional dynamics of pairs of distal DNA loci on a compacted chromosome.bioRxiv : the preprint server for biology · 2023Article
- DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function.eLife · 2022Article
- Regulation of the mitotic chromosome folding machines.The Biochemical journal · 2022Article
- A Versatile Micromanipulation Apparatus for Biophysical Assays of the Cell Nucleus.Cellular and molecular bioengineering · 2022Article
- Force by minus-end motors Dhc1 and Klp2 collapses the S. pombe spindle after laser ablation.Biophysical journal · 2022Article
- A survey of physical methods for studying nuclear mechanics and mechanobiology.APL bioengineering · 2021Review
- Article
- DNA-Topology Simplification by Topoisomerases.Molecules (Basel, Switzerland) · 2021Review
- Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly.Nature communications · 2021Article
- Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields.Molecular cell · 2020Review
- Topoisomerase IIα is essential for maintenance of mitotic chromosome structure.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
Abstract
We have analyzed the topological organization of chromatin inside mitotic chromosomes. We show that mitotic chromatin is heavily self-entangled through experiments in which topoisomerase (topo) II is observed to reduce mitotic chromosome elastic stiffness. Single chromosomes were relaxed by 35% by exogenously added topo II in a manner that depends on hydrolysable adenosine triphosphate (ATP), whereas an inactive topo II cleavage mutant did not change chromosome stiffness. Moreover, experiments using type I topos produced much smaller relaxation effects than topo II, indicating that chromosome relaxation by topo II is caused by decatenation and/or unknotting of double-stranded DNA. In further experiments in which chromosomes are first exposed to protease to partially release protein constraints on chromatin, ATP alone relaxes mitotic chromosomes. The topo II-specific inhibitor ICRF-187 blocks this effect, indicating that it is caused by endogenous topo II bound to the chromosome. Our experiments show that DNA entanglements act in concert with protein-mediated compaction to fold chromatin into mitotic chromosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.