ArticleThe Journal of biological chemistry2010
T cell signal regulation by the actin cytoskeleton.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Codependencies of mTORC1 signaling and endolysosomal actin structures.Science advances · 2023Article
- Article
- Emerging roles of cytoskeletal proteins in regulating gene expression and genome organization during differentiation.Nucleus (Austin, Tex.) · 2020Review
- T-Cell Mechanobiology: Force Sensation, Potentiation, and Translation.Frontiers in physics · 2019Article
- Triggering signaling pathways using F-actin self-organization.Scientific reports · 2016Article
- n-3 polyunsaturated fatty acids suppress CD4(+) T cell proliferation by altering phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] organization.Biochimica et biophysica acta · 2016Article
- The T cell receptor resides in ordered plasma membrane nanodomains that aggregate upon patching of the receptor.Scientific reports · 2015Article
- Neutral sphingomyelinase in physiological and measles virus induced T cell suppression.PLoS pathogens · 2014Article
- Interaction of membrane/lipid rafts with the cytoskeleton: impact on signaling and function: membrane/lipid rafts, mediators of cytoskeletal arrangement and cell signaling.Biochimica et biophysica acta · 2014Review
- CD28 sensitizes TCR Ca²⁺ signaling during Ag-independent polarization of plasma membrane rafts.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- CD81 controls sustained T cell activation signaling and defines the maturation stages of cognate immunological synapses.Molecular and cellular biology · 2013Article
- Cytoskeletal modulation of lipid interactions regulates Lck kinase activity.The Journal of biological chemistry · 2012Article
- Spleen tyrosine kinase (Syk) is a potent target for GvHD prevention at different cellular levels.Leukemia · 2012Article
- Annexins as organizers of cholesterol- and sphingomyelin-enriched membrane microdomains in Niemann-Pick type C disease.Cellular and molecular life sciences : CMLS · 2012Review
- Lck, Membrane Microdomains, and TCR Triggering Machinery: Defining the New Rules of Engagement.Frontiers in immunology · 2012Article
- How does the kinase Lck phosphorylate the T cell receptor? Spatial organization as a regulatory mechanism.Frontiers in immunology · 2012Article
- Cytoplasmic domain of P-selectin glycoprotein ligand-1 facilitates dimerization and export from the endoplasmic reticulum.The Journal of biological chemistry · 2011Article
- Retroviral matrix and lipids, the intimate interaction.Retrovirology · 2011Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
T cells form an immunological synapse (IS) that sustains and regulates signals for cell stimulation. Enriched in the IS is the Src family kinase Lck. Conversely, the membrane phosphatase CD45, which activates Src family kinases, is excluded, and this is necessary to avoid quenching of T cell receptor phosphosignals. Data suggest that this arrangement occurs by an enrichment of cholesterol-dependent rafts in the IS. However, the role of rafts in structuring the IS remains unclear. To address this question, we used fluorescence resonance energy transfer (FRET) to interrogate the nanoscopic structure of the IS. The FRET probes consisted of membrane-anchored fluorescent proteins with distinct affinities for rafts. Both the raft and nonraft probes exhibited clustering in the IS. However, co-clustering of raft donor-acceptor pairs was 10-fold greater than co-clustering of raft-nonraft pairs. We measured the effect of disrupting rafts in the IS on CD45 localization and Lck regulation by treating stimulated T cells with filipin. The filipin specifically disrupted co-clustering of the raft FRET pairs in the IS and allowed targeting of CD45 to the IS and dephosphorylation of the regulatory tyrosine of Lck. Clustering of the raft probes was also sensitive to latrunctulin B, which disrupts actin filaments. Strikingly, enriching the cortical cytoskeleton using jasplakinolide maintained raft probe co-clustering, CD45 exclusion, and Lck regulation in the IS following the addition of filipin. These data show the actin cytoskeleton maintains a membrane raft environment in the IS that promotes Lck regulation by excluding CD45.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.