ArticleInfection and immunity1991
Elevation of intracellular free calcium levels in HEp-2 cells infected with enteropathogenic Escherichia coli.
Article in Infection and immunity, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 161 citations in OpenAlex.
- Role of the eaeA gene in experimental enteropathogenic Escherichia coli infection.The Journal of clinical investigation · 1993Trial
- EnteropathogeniceLife · 2026Article
- The EHEC-host interactome reveals novel targets for the translocated intimin receptor.Scientific reports · 2014Article
- Impact of calcium signaling during infection of Neisseria meningitidis to human brain microvascular endothelial cells.PloS one · 2014Article
- IQGAP1 in microbial pathogenesis: Targeting the actin cytoskeleton.FEBS letters · 2011Review
- Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli.Frontiers in microbiology · 2011Article
- Actin pedestal formation by enteropathogenic Escherichia coli is regulated by IQGAP1, calcium, and calmodulin.The Journal of biological chemistry · 2008Article
- Pathogenicity islands in bacterial pathogenesis.Clinical microbiology reviews · 2004Review
- Calpain regulates enterocyte brush border actin assembly and pathogenic Escherichia coli-mediated effacement.The Journal of biological chemistry · 2003Article
- Characterization of the novel factor paa involved in the early steps of the adhesion mechanism of attaching and effacing Escherichia coli.Infection and immunity · 2003Article
- Virulence of enteropathogenic Escherichia coli, a global pathogen.Clinical microbiology reviews · 2003Review
- Differential modulation by Ca2+ of type III secretion of diffusely adhering enteropathogenic Escherichia coli.Infection and immunity · 2003Article
- Mycoplasma hyopneumoniae increases intracellular calcium release in porcine ciliated tracheal cells.Infection and immunity · 2002Article
- Rapid activation of protein tyrosine kinase and phospholipase C-gamma2 and increase in cytosolic free calcium are required by Ehrlichia chaffeensis for internalization and growth in THP-1 cells.Infection and immunity · 2002Article
- Myosin ii light chain phosphorylation regulates membrane localization and apoptotic signaling of tumor necrosis factor receptor-1.The Journal of biological chemistry · 2001Article
- Implication of mitogen-activated protein kinases in T84 cell responses to enteropathogenic Escherichia coli infection.Infection and immunity · 2001Article
- Salmonella typhimurium induces epithelial IL-8 expression via Ca(2+)-mediated activation of the NF-kappaB pathway.The Journal of clinical investigation · 2000Article
- Listeria monocytogenes phospholipase C-dependent calcium signaling modulates bacterial entry into J774 macrophage-like cells.Infection and immunity · 1999Article
- Enteropathogenic Escherichia coli inhibits phagocytosis.Infection and immunity · 1999Article
- Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis.Microbiology and molecular biology reviews : MMBR · 1998Review
7 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Enteropathogenic Escherichia coli (EPEC) are a class of diarrheagenic organisms that induce a characteristic attaching and effacing lesion in enterocytes and various cultured cell lines. Infection of cultured HEp-2 cells by EPEC isolates 2036-80 (serotype O119) and E2348-69 (serotype O127) resulted in significant elevation of intracellular free calcium levels, determined quantitatively with the fluorescent calcium indicator dye 2-([2-bis(carboxymethyl)amino-5-methylphenoxy]methyl)-6-methoxy-8- bis(carboxymethyl)aminoquinoline. This effect, which was not observed on infection with non-lesion-forming E. coli strains, was inhibited by dantrolene, a drug that prevents calcium mobilization from intracellular stores. Moreover, activated protein kinase C in infected cells was dissociated from cell membranes by a process that was inhibited by cyclosporin A, suggesting involvement of the calcium-dependent protease calpain. A qualitative method for observing intracellular calcium fluxes by fluorescence microscopy with the recently described fluorescein-based indicator fluo-3 was used to screen a collection of well-characterized E. coli isolates from patients with infantile enteritis. Increased localized calcium-dependent fluo-3 fluorescence was observed only in HEp-2 cells infected with known lesion-forming EPEC strains. We propose that enhancement of intracellular free calcium levels in enterocytes infected with EPEC would result in formation of the characteristic lesion by calcium-dependent activation of actin-depolymerizing proteins, with eventual loss of absorptive capacity.
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