ArticleMutation research2010
DNA methylation changes in a human cell model of breast cancer progression.
Article in Mutation research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Epigenetic reprogramming of lineage switching in cancer.FEBS letters · 2026Review
- Identification of STXBP6-IRF1 positive feedback loop in regulation of PD-L1 in cancer.Cancer immunology, immunotherapy : CII · 2021Article
- NRG1 fusions in breast cancer.Breast cancer research : BCR · 2021Article
- circ_0002346 Suppresses Non-Small-Cell Lung Cancer Progression Depending on the Regulation of the miR-582-3p/STXBP6 Axis.International journal of genomics · 2021Article
- PI(4,5)PProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Modification of the association between recreational physical activity and survival after breast cancer by promoter methylation in breast cancer-related genes.Breast cancer research : BCR · 2017Article
- Identification of Methylation-Driven, Differentially Expressed STXBP6 as a Novel Biomarker in Lung Adenocarcinoma.Scientific reports · 2017Article
- DNA methylation modifies the association between obesity and survival after breast cancer diagnosis.Breast cancer research and treatment · 2016Article
- The mammary gland carcinogens: the role of metal compounds and organic solvents.International journal of breast cancer · 2013Article
- Cardiac side effects of anticancer treatments: new mechanistic insights.Current heart failure reports · 2012Review
- A Study of the Association between SNP8NRG241930 in the 5' End of Neuroglin 1 Gene with Schizophrenia in a Group of Iranian Patients.Cell journal · 2011Article
- Metabolomic profiling reveals a role for androgen in activating amino acid metabolism and methylation in prostate cancer cells.PloS one · 2011Article
- Reactivation of epigenetically silenced HER4/ERBB4 results in apoptosis of breast tumor cells.Oncogene · 2010Article
- Gene-Specific Promoter Methylation Status in Hormone-Receptor-Positive Breast Cancer Associates with Postmenopausal Body Size and Recreational Physical Activity.International journal of cancer and clinical researchArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Epigenetic inactivation of genes by DNA hypermethylation plays an important role in carcinogenesis. An in vitro model of human breast epithelial cell transformation was used to study epigenetic changes induced by estradiol during the neoplastic process. Different stages of tumor initiation and progression are represented in this model being MCF-10F the normal stage; trMCF cells, the transformed stage; bsMCF cells, the invasive stage and, caMCF cells, the tumor stage. Global methylation studies by restriction landmark genomic scanning (RLGS) showed an increased DNA methylation during the in the invasive and tumor stages. Expression studies showed that NRG1 (neuregulin 1), CSS3 (chondroitin sulfate synthase 3) and SNIP (SNAP-25-interacting protein) were downregulated in the invasive and tumor cells. The transformed cells showed low expression of STXBP6 (amysin) compared to the parental cells MCF-10F. The treatment of these cells with the demethylating agent 5-aza-dC alone or in combination with the histone deacetylase inhibitor trichostatin increased the expression of NRG1, STXBP6, CSS3 and SNIP confirming that DNA methylation plays an important role in the regulation of the expression of these genes. The NRG1 exon 1 has a region located between -136 and +79 (considering +1, the translational initiation site) rich in CpG sites that was analyzed by methylation specific PCR (MSP). NRG1 exon 1 showed progressive changes in the methylation pattern associated with the progression of the neoplastic process in this model; NRG1 exon 1 was unmethylated in MCF-10F and trMCF cells, becoming hypermethylated in the invasive (bsMCF) and tumor (caMCF) stages. Studies of human breast tissue samples showed that NRG1 exon 1 was partially methylated in 14 out of 17 (82.4%) invasive carcinomas although it was unmethylated in normal tissues (8 out of 10 normal breast tissue samples). Furthermore, NRG1 exon 1 was partially methylated in 9 out of 14 (64.3%) morphologically normal tissue samples adjacent to invasive carcinomas.
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