ArticleAnalytical biochemistry2010
Maximum yields of microsomal-type membranes from small amounts of plant material without requiring ultracentrifugation.
Article in Analytical biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed.
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- In vitro thyroperoxidase inhibition assessment by LC-ICP-MS-based L-tyrosine iodination assay: comparison with Amplex Ultrared assay and its modifications.Archives of toxicology · 2026Article
- Dual ubiquitin signalling by SLOMO controls AUX1 activity and turnover during root gravitropism.The EMBO journal · 2026Article
- LRRK2 regulates ArfGAP1 membrane localization, activity and neuronal toxicity via phosphorylation within its lipid-sensing ALPS2 motif.bioRxiv : the preprint server for biology · 2026Article
- LRRK2 regulates ArfGAP1 membrane localization, activity and neuronal integrity via phosphorylation within its lipid-sensing ALPS2 motif.Frontiers in molecular neuroscience · 2026Article
- Complex sphingolipid metabolism impacts cell division and plasmodesmal development in the moss Physcomitrium patens.Plant physiology · 2025Article
- A plant-specific cytochromeScience advances · 2025Article
- Mutated sigma-1R disrupts cell homeostasis in dHMN patient cells.Cellular and molecular life sciences : CMLS · 2025Article
- Identification of INOSITOL PHOSPHORYLCERAMIDE SYNTHASE 2 (IPCS2) as a new rate-limiting component in Arabidopsis pathogen entry control.The Plant journal : for cell and molecular biology · 2025Article
- The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum-Plasma Membrane Contact Sites and Interacts With AVRPM3Molecular plant pathology · 2025Article
- Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties.Frontiers in plant science · 2025Article
- Tetraspanin 5 orchestrates resilience to salt stress through the regulation of ion and reactive oxygen species homeostasis in rice.Plant biotechnology journal · 2025Article
- Tissue-specific accumulation of PIP aquaporins of a particular heteromeric composition is part of the maize response to mycorrhiza and drought.Scientific reports · 2024Article
- Article
- Fine Structure of Plasmodesmata-Associated Membrane Bodies Formed by Viral Movement Protein.Plants (Basel, Switzerland) · 2023Article
- Nt-acetylation-independent turnover of SQUALENE EPOXIDASE 1 by Arabidopsis DOA10-like E3 ligases.Plant physiology · 2023Article
- Resurrection of plant disease resistance proteins via helper NLR bioengineering.Science advances · 2023Article
- Dynamic GOLVEN-ROOT GROWTH FACTOR 1 INSENSITIVE signaling in the root cap mediates root gravitropism.Plant physiology · 2023Article
- BRASSINOSTEROID INSENSITIVE1 internalization can occur independent of ligand binding.Plant physiology · 2023Article
- Effector-dependent activation and oligomerization of plant NRC class helper NLRs by sensor NLR immune receptors Rpi-amr3 and Rpi-amr1.The EMBO journal · 2023Article
18 more citing papers are in PubMed but not listed here.
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2 authors.
Funding
Abstract
Isolation of a microsomal membrane fraction is a common procedure in studies involving membrane proteins. By conventional definition, microsomal membranes are collected by centrifugation of a postmitochondrial fraction at 100,000g in an ultracentrifuge, a method originally developed for large amounts of mammalian tissue. We present a method for isolating microsomal-type membranes from small amounts of Arabidopsis thaliana plant material that does not rely on ultracentrifugation but instead uses the lower relative centrifugal force (21,000g) of a microcentrifuge. We show that the 21,000g pellet is equivalent to that obtained at 100,000g and that it contains all of the membrane fractions expected in a conventional microsomal fraction. Our method incorporates specific manipulation of sample density throughout the procedure, with minimal preclearance, minimal volumes of extraction buffer, and minimal sedimentation pathlength. These features allow maximal membrane yields, enabling membrane isolation from limited amounts of material. We further demonstrate that conventional ultracentrifuge-based protocols give submaximal yields due to losses during early stages of the procedure; that is, extensive amounts of microsomal-type membranes can sediment prematurely during the typical preclearance steps. Our protocol avoids such losses, thereby ensuring maximal yield and a representative total membrane fraction. The principles of our method can be adapted for nonplant material.
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