ArticleApplied and environmental microbiology2010
Identification of Bacillus cereus genes specifically expressed during growth at low temperatures.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
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- Morphological Features and Cold-Response Gene Expression in MesophilicMicroorganisms · 2021Article
- Metagenomics and Culture Dependent Insights into the Distribution of Firmicutes across Two Different Sample Types Located in the Black Hills Region of South Dakota, USA.Microorganisms · 2021Article
- Short-Chain and Unsaturated Fatty Acids Increase Sequentially From the Lag Phase During Cold Growth ofFrontiers in microbiology · 2021Article
- Bacillus cereusApplied and environmental microbiology · 2019Article
- Genes Associated With PsychrotolerantFrontiers in microbiology · 2019Article
- Identification of Fatty Acids in Bacillus cereus.Journal of visualized experiments : JoVE · 2016Article
- Fatty acid profiles and desaturase-encoding genes are different in thermo- and psychrotolerant strains of the Bacillus cereus Group.BMC research notes · 2015Article
- Proteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors.Frontiers in microbiology · 2015Article
- The Water Cycle, a Potential Source of the Bacterial Pathogen Bacillus cereus.BioMed research international · 2015Article
- Role of fatty acids in Bacillus environmental adaptation.Frontiers in microbiology · 2015Review
- The CasKR two-component system is required for the growth of mesophilic and psychrotolerant Bacillus cereus strains at low temperatures.Applied and environmental microbiology · 2014Article
- Glucose 6P binds and activates HlyIIR to repress Bacillus cereus haemolysin hlyII gene expression.PloS one · 2013Article
- Involvement of two-component system CBO0366/CBO0365 in the cold shock response and growth of group I (proteolytic) Clostridium botulinum ATCC 3502 at low temperatures.Applied and environmental microbiology · 2012Article
- Influence of anaerobiosis and low temperature on Bacillus cereus growth, metabolism, and membrane properties.Applied and environmental microbiology · 2012Article
- Production of protocatechuic acid in Bacillus Thuringiensis ATCC33679.International journal of molecular sciences · 2012Article
- Differential involvement of the five RNA helicases in adaptation of Bacillus cereus ATCC 14579 to low growth temperatures.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms involved in the ability of Bacillus cereus to multiply at low temperatures were investigated. It was assumed that many genes involved in cold acclimation would be upregulated at low temperatures. Recombinase-based in vivo expression technology (IVET) was adapted to the detection of the transient activation of B. cereus promoters during growth at 10 degrees C. Four independent screenings of a promoter library from type strain ATCC 14579 were performed, and 17 clones were isolated. They corresponded to 17 promoter regions that displayed reproducibly elevated expression at 10 degrees C relative to expression at 30 degrees C. This analysis revealed several genes that may be important for B. cereus to grow successfully under the restrictive conditions of cold habitats. Among them, a locus corresponding to open reading frames BC5402 to BC5398, harboring a lipase-encoding gene and a putative transcriptional regulator, was identified three times. While a mutation in the putative regulator-encoding gene did not cause any particular phenotype, a mutant deficient in the lipase-encoding gene showed reduced growth abilities at low temperatures compared with the parental strain. The mutant did not change its fatty acid profiles in the same way as the wild type when grown at 12 degrees C instead of 37 degrees C. This study demonstrates the feasibility of a promoter trap strategy for identifying cold-induced genes. It outlines a first picture of the different processes involved in B. cereus cold acclimation.
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