ArticleApplied and environmental microbiology2010
Detection and genetic analysis of human sapoviruses in river water in Japan.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Gastrointestinal tract and viral pathogens.World journal of virology · 2023Review
- Detection of Human Sapoviruses in Sewage in China by Next Generation Sequencing.Food and environmental virology · 2021Article
- Human sapovirus propagation in human cell lines supplemented with bile acids.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses.Archives of virology · 2020Article
- Review
- Article
- Occurrence of microbial indicators, pathogenic bacteria and viruses in tropical surface waters subject to contrasting land use.Water research · 2019Article
- Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters.Scientific reports · 2018Article
- Sapovirus in Wastewater Treatment Plants in Tunisia: Prevalence, Removal, and Genetic Characterization.Applied and environmental microbiology · 2018Article
- Cellulose-based virus-retentive filters: a review.Re/views in environmental science and bio/technology · 2017Review
- Human Sapovirus in Mussels from Ría do Burgo, A Coruña (Spain).Food and environmental virology · 2016Article
- Prevalence and Genetic Diversity of Human Sapoviruses in Shellfish from Commercial Production Areas in Galicia, Spain.Applied and environmental microbiology · 2016Article
- Wastewater Analysis Indicates that Genetically Diverse Astroviruses, Including Strains Belonging to Novel Clades MLB and VA, Are Circulating within Japanese Populations.Applied and environmental microbiology · 2015Article
- Comprehensive review of human sapoviruses.Clinical microbiology reviews · 2015Review
- Article
- Detection of multiple human sapoviruses from imported frozen individual clams.Food and environmental virology · 2013Article
- Application of acidic elution to virus concentration using electropositive filters.Food and environmental virology · 2013Article
- Application of a competitive internal amplification control for the detection of sapoviruses in wastewater.Food and environmental virology · 2013Article
- Internalization of sapovirus, a surrogate for norovirus, in romaine lettuce and the effect of lettuce latex on virus infectivity.Applied and environmental microbiology · 2012Article
- Occurrence of viruses and protozoa in drinking water sources of Japan and their relationship to indicator microorganisms.Food and environmental virology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the prevalence of sapoviruses (SaVs) in the Tamagawa River in Japan from April 2003 to March 2004 and performed genetic analysis of the SaV genes identified in river water. A total of 60 river water samples were collected from five sites along the river, and 500 ml was concentrated using the cation-coated filter method. By use of a real-time reverse transcription (RT)-PCR assay, 12 (20%) of the 60 samples were positive for SaV. SaV sequences were obtained from 15 (25%) samples, and a total of 30 SaV strains were identified using six RT-PCR assays followed by cloning and sequence analysis. A newly developed nested RT-PCR assay utilizing a broadly reactive forward primer showed the highest detection efficiency and amplified more diverse SaV genomes in the samples. SaV sequences were frequently detected from November to March, whereas none were obtained in April, July, September, or October. No SaV sequences were detected in the upstream portion of the river, whereas the midstream portion showed high positive rates. Based on phylogenetic analysis, SaV strains identified in the river water samples were classified into nine genotypes, namely, GI/1, GI/2, GI/3, GI/5, GI/untyped, GII/1, GII/2, GII/3, and GV/1. To our knowledge, this is the first study describing seasonal and spatial distributions and genetic diversity of SaVs in river water. A combination of real-time RT-PCR assay and newly developed nested RT-PCR assay is useful for identifying and characterizing SaV strains in a water environment.
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