ArticleJournal of the American Society for Mass Spectrometry2010
Confident assignment of intact mass tags to human salivary cystatins using top-down Fourier-transform ion cyclotron resonance mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Salivary Cystatin D Interactome in Patients with Systemic Mastocytosis: An Exploratory Study.International journal of molecular sciences · 2023Article
- The Post-Translational Modifications of Human Salivary Peptides and Proteins Evidenced by Top-Down Platforms.International journal of molecular sciences · 2023Review
- Comparison of Protein Variation inToxins · 2022Article
- Specimen-specific drift of densities defines distinct subclasses of extracellular vesicles from human whole saliva.PloS one · 2021Article
- RP-HPLC-ESI-IT Mass Spectrometry Reveals Significant Variations of the Human Salivary Protein Profile Associated with Predominantly Antibody Deficiencies.Journal of clinical immunology · 2020Article
- Targeted Proteomics Guided by Label-free Quantitative Proteome Analysis in Saliva Reveal Transition Signatures from Health to Periodontal Disease.Molecular & cellular proteomics : MCP · 2018Article
- More Accurate Oral Cancer Screening with Fewer Salivary Biomarkers.Biomarkers in cancer · 2017Article
- Protein-species quantitative venomics: looking through a crystal ball.The journal of venomous animals and toxins including tropical diseases · 2017Review
- Progress in Top-Down Proteomics and the Analysis of Proteoforms.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Review
- Evidence of Alternative Cystatin C Signal Sequence Cleavage Which Is Influenced by the A25T Polymorphism.PloS one · 2016Article
- Significant modifications of the salivary proteome potentially associated with complications of Down syndrome revealed by top-down proteomics.Molecular & cellular proteomics : MCP · 2013Article
- Top-down targeted proteomics for deep sequencing of tropomyosin isoforms.Journal of proteome research · 2013Article
- Impairment of mitochondria in adult mouse brain overexpressing predominantly full-length, N-terminally acetylated human α-synuclein.PloS one · 2013Article
- Top-Down Characterization of the Post-Translationally Modified Intact Periplasmic Proteome from the Bacterium Novosphingobium aromaticivorans.International journal of proteomics · 2013Article
- Defining intact protein primary structures from saliva: a step toward the human proteome project.Analytical chemistry · 2012Article
- Electrochemistry-assisted top-down characterization of disulfide-containing proteins.Analytical chemistry · 2012Article
- Profiling of integral membrane proteins and their post translational modifications using high-resolution mass spectrometry.Methods (San Diego, Calif.) · 2011Review
- Dividing to unveil protein microheterogeneities: traveling wave ion mobility study.Analytical chemistry · 2011Article
- Analysis of intact protein isoforms by mass spectrometry.The Journal of biological chemistry · 2011Review
- Salivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study.Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicalsArticle
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
A hybrid linear ion-trap Fourier-transform ion cyclotron resonance mass spectrometer was used for top-down characterization of the abundant human salivary cystatins, including S, S1, S2, SA, SN, C, and D, using collisionally activated dissociation (CAD) after chromatographic purification of the native, disulfide intact proteins. Post-translational modifications and protein sequence polymorphisms arising from single nucleotide polymorphisms (SNPs) were assigned from precursor and product ion masses at a tolerance of 10 ppm, allowing confident identification of individual intact mass tags. Cystatins S, S1, S2, SA, and SN were cleaved of a N-terminal 20 amino acid signal peptide and cystatin C a 26-residue peptide, to yield a generally conserved N-terminus. In contrast, cystatin D isoforms with 24 and 28 amino acid residue N-terminal truncations were found such that their N-termini were not conserved. Cystatin S1 was phosphorylated at Ser3, while S2 was phosphorylated at Ser1 and Ser3, in agreement with previous work. Both cystatin D isoforms carried the polymorphism C46R (SNP: rs1799841). The 14,328 Da isoform of cystatin SN previously assigned with polymorphism P31L due to a SNP (rs2070856) was found only in whole saliva. Parotid secretions contained no detectable cystatins while whole saliva largely mirrored the contents of submandibular/sublingual (SMSL) secretions. With fully characterized cystatin intact mass tags it will now be possible to examine the correlation between the abundance of these molecules and human health and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.