ArticleProteome science2010
Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots.
Article in Proteome science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- Fructose biphosphate aldolase: A new cassava allergen.The World Allergy Organization journal · 2023Article
- A population based expression atlas provides insights into disease resistance and other physiological traits in cassava (Manihot esculenta Crantz).Scientific reports · 2021Article
- Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family MemberPlants (Basel, Switzerland) · 2021Article
- Old Catharanthus roseus culture (14 years) produced somatic embryos and plants and showed normal genome size; demonstrated an increased antioxidant defense mechanism; and synthesized stress proteins as biochemical, proteomics, and flow-cytometry studies reveal.Journal of applied genetics · 2021Article
- Endophytic bacterial and fungal microbiota in different cultivars of cassava (Manihot esculenta Crantz).Journal of microbiology (Seoul, Korea) · 2020Article
- Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA).Scientific reports · 2020Article
- ArabidopsisFrontiers in plant science · 2019Article
- Prediction of cassava protein interactome based on interolog method.Scientific reports · 2017Article
- Gene expression atlas for the food security crop cassava.The New phytologist · 2017Article
- Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities.Frontiers in plant science · 2017Review
- Proteomics Profiling Reveals Carbohydrate Metabolic Enzymes and 14-3-3 Proteins Play Important Roles for Starch Accumulation during Cassava Root Tuberization.Scientific reports · 2016Article
- The Comparatively Proteomic Analysis in Response to Cold Stress in Cassava Plantlets.Plant molecular biology reporter · 2016Article
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- Cassava root membrane proteome reveals activities during storage root maturation.Journal of plant research · 2016Article
- Global scale transcriptome analysis of Arabidopsis embryogenesis in vitro.BMC genomics · 2015Article
- Analysis of different strategies adapted by two cassava cultivars in response to drought stress: ensuring survival or continuing growth.Journal of experimental botany · 2015Article
- Comparison of leaf proteomes of cassava (Manihot esculenta Crantz) cultivar NZ199 diploid and autotetraploid genotypes.PloS one · 2014Article
- Starch biosynthesis in cassava: a genome-based pathway reconstruction and its exploitation in data integration.BMC systems biology · 2013Article
- Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar.Proteome science · 2011Article
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Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProteomics is increasingly becoming an important tool for the study of many different aspects of plant functions, such as investigating the molecular processes underlying in plant physiology, development, differentiation and their interaction with the environments. To investigate the cassava (Manihot esculenta Crantz) proteome, we extracted proteins from somatic embryos, plantlets and tuberous roots of cultivar SC8 and separated them by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
resultsAnalysis by liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) yielded a total of 383 proteins including isoforms, classified into 14 functional groups. The majority of these were carbohydrate and energy metabolism associated proteins (27.2%), followed by those involved in protein biosynthesis (14.4%). Subsequent analysis has revealed that 54, 59, 74 and 102 identified proteins are unique to the somatic embryos, shoots, adventitious roots and tuberous roots, respectively. Some of these proteins may serve as signatures for the physiological and developmental stages of somatic embryos, shoots, adventitious roots and tuberous root. Western blotting results have shown high expression levels of Rubisco in shoots and its absence in the somatic embryos. In addition, high-level expression of alpha-tubulin was found in tuberous roots, and a low-level one in somatic embryos. This extensive study effectively provides a huge data set of dynamic protein-related information to better understand the molecular basis underlying cassava growth, development, and physiological functions.
conclusionThis work paves the way towards a comprehensive, system-wide analysis of the cassava. Integration with transcriptomics, metabolomics and other large scale "-omics" data with systems biology approaches can open new avenues towards engineering cassava to enhance yields, improve nutritional value and overcome the problem of post-harvest physiological deterioration.
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Registered trials
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