ArticleJournal of biological engineering2010
A modular positive feedback-based gene amplifier.
Article in Journal of biological engineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Programming Next-Generation Synthetic Biosensors by Genetic Circuit Design.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Genetic "expiry-date" circuits control lifespan of synthetic scavenger bacteria for safe bioremediation.Nucleic acids research · 2025Article
- Biological Switches: Past and Future Milestones of Transcription Factor-Based Biosensors.ACS synthetic biology · 2025Review
- AFrontiers in cardiovascular medicine · 2024Article
- Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier.Synthetic and systems biotechnology · 2023Article
- Advances in Synthetic-Biology-Based Whole-Cell Biosensors: Principles, Genetic Modules, and Applications in Food Safety.International journal of molecular sciences · 2023Review
- Synthetic bacteria for the detection and bioremediation of heavy metals.Frontiers in bioengineering and biotechnology · 2023Review
- Characterizing a New Fluorescent Protein for a Low Limit of Detection Sensing in the Cell-Free System.ACS synthetic biology · 2022Article
- Construction of cadmium whole-cell biosensors and circuit amplification.Applied microbiology and biotechnology · 2021Article
- Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit.Frontiers in bioengineering and biotechnology · 2021Article
- A CRISPR-Cas autocatalysis-driven feedback amplification network for supersensitive DNA diagnostics.Science advances · 2021Article
- Synthetic Biology Enables Programmable Cell-Based Biosensors.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Review
- Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection.Applied and environmental microbiology · 2019Article
- Development of Aspirin-Inducible Biosensors inApplied and environmental microbiology · 2019Article
- Intracellular Signaling by themSphere · 2018Article
- Tailor-made transcriptional biosensors for optimizing microbial cell factories.Journal of industrial microbiology & biotechnology · 2017Review
- Synthetic Biology: Engineering Living Systems from Biophysical Principles.Biophysical journal · 2017Review
- Designer cell signal processing circuits for biotechnology.New biotechnology · 2015Review
- In Vivo Programmed Gene Expression Based on Artificial Quorum Networks.Applied and environmental microbiology · 2015Article
- A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine.Microbial cell factories · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPositive feedback is a common mechanism used in the regulation of many gene circuits as it can amplify the response to inducers and also generate binary outputs and hysteresis. In the context of electrical circuit design, positive feedback is often considered in the design of amplifiers. Similar approaches, therefore, may be used for the design of amplifiers in synthetic gene circuits with applications, for example, in cell-based sensors.
resultsWe developed a modular positive feedback circuit that can function as a genetic signal amplifier, heightening the sensitivity to inducer signals as well as increasing maximum expression levels without the need for an external cofactor. The design utilizes a constitutively active, autoinducer-independent variant of the quorum-sensing regulator LuxR. We experimentally tested the ability of the positive feedback module to separately amplify the output of a one-component tetracycline sensor and a two-component aspartate sensor. In each case, the positive feedback module amplified the response to the respective inducers, both with regards to the dynamic range and sensitivity.
conclusionsThe advantage of our design is that the actual feedback mechanism depends only on a single gene and does not require any other modulation. Furthermore, this circuit can amplify any transcriptional signal, not just one encoded within the circuit or tuned by an external inducer. As our design is modular, it can potentially be used as a component in the design of more complex synthetic gene circuits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.