ArticleProtoplasma2010
Subcellular compartmentation of glutathione in dicotyledonous plants.
Article in Protoplasma, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 96 citations in OpenAlex.
- Article
- Analysis on morphological characteristics and identification of candidate genes during the flowering development of alfalfa.Frontiers in plant science · 2024Article
- Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis.Antioxidants (Basel, Switzerland) · 2023Review
- How Extracellular Reactive Oxygen Species Reach Their Intracellular Targets in Plants.Molecules and cells · 2023Review
- Antioxidant Potential of Glutathione and Crosstalk with Phytohormones in Enhancing Abiotic Stress Tolerance in Crop Plants.Plants (Basel, Switzerland) · 2023Review
- Enzymatic Investigation ofProteomes · 2023Article
- Organelle-specific localization of glutathione in plants grown under different light intensities and spectra.Histochemistry and cell biology · 2022Review
- Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione inInternational journal of molecular sciences · 2021Article
- Chemical Composition, Antioxidant and Anticancer Activities ofMolecules (Basel, Switzerland) · 2020Article
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Glutathione redox state plays a key role in flower development and pollen vigour.Journal of experimental botany · 2020Article
- Article
- Thiol Based Redox Signaling in Plant Nucleus.Frontiers in plant science · 2018Review
- Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance.Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Review
- A comparative analysis of transcriptomic, biochemical, and physiological responses to elevated ozone identifies species-specific mechanisms of resilience in legume crops.Journal of experimental botany · 2015Article
- The uptake and bioaccumulation of heavy metals by food plants, their effects on plants nutrients, and associated health risk: a review.Environmental science and pollution research international · 2015Review
- Compartment specific response of antioxidants to drought stress in Arabidopsis.Plant science : an international journal of experimental plant biology · 2014Article
- Higher sensitivity of pad2-1 and vtc2-1 mutants to cadmium is related to lower subcellular glutathione rather than ascorbate contents.Protoplasma · 2014Article
- Compartment-specific importance of glutathione during abiotic and biotic stress.Frontiers in plant science · 2014Article
- Transcriptomics of desiccation tolerance in the streptophyte green alga Klebsormidium reveal a land plant-like defense reaction.PloS one · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This study describes the subcellular distribution of glutathione in roots and leaves of different plant species (Arabidopsis, Cucurbita, and Nicotiana). Glutathione is an important antioxidant and redox buffer which is involved in many metabolic processes including plant defense. Thus information on the subcellular distribution in these model plants especially during stress situations provides a deeper insight into compartment specific defense reactions and reflects the occurrence of compartment specific oxidative stress. With immunogold cytochemistry and computer-supported transmission electron microscopy glutathione could be localized in highest contents in mitochondria, followed by nuclei, peroxisomes, the cytosol, and plastids. Within chloroplasts and mitochondria, glutathione was restricted to the stroma and matrix, respectively, and did not occur in the lumen of cristae and thylakoids. Glutathione was also found at the membrane and in the lumen of the endoplasmic reticulum. It was also associated with the trans and cis side of dictyosomes. None or only very little glutathione was detected in vacuoles and the apoplast of mesophyll and root cells. Additionally, glutathione was found in all cell compartments of phloem vessels, vascular parenchyma cells (including vacuoles) but was absent in xylem vessels. The specificity of this method was supported by the reduction of glutathione labeling in all cell compartments (up to 98%) of the glutathione-deficient Arabidopsis thaliana rml1 mutant. Additionally, we found a similar distribution of glutathione in samples after conventional fixation and rapid microwave-supported fixation. Thus, indicating that a redistribution of glutathione does not occur during sample preparation. Summing up, this study gives a detailed insight into the subcellular distribution of glutathione in plants and presents solid evidence for the accuracy and specificity of the applied method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.