ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2010
Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem cells that form organoids in vitro.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 152 citations in OpenAlex.
- Epigenetic reprogramming of lineage switching in cancer.FEBS letters · 2026Review
- Intestinal epithelial TLR5 signaling promotes barrier-supportive macrophages.Science immunology · 2026Article
- Tet1 safeguards lineage allocation in intestinal stem cells.bioRxiv : the preprint server for biology · 2025Article
- Article
- Lgr5 + intestinal stem cells are required for organoid survival after genotoxic injury.Development (Cambridge, England) · 2024Article
- Chronic arsenic exposure affects stromal cells and signaling in the small intestine in a sex-specific manner.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- A new microphysiological system shows hypoxia primes human ISCs for interleukin-dependent rescue of stem cell activity.bioRxiv : the preprint server for biology · 2023Article
- Single-Cell and Spatial Analysis of Emergent Organoid Platforms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Cellular and transcriptional heterogeneity in the intrahepatic biliary epithelium.Gastro hep advances · 2023Article
- The lncRNA Tincr Regulates the Abnormal Differentiation of Intestinal Epithelial Stem Cells in the Diabetic StateCurrent stem cell research & therapy · 2023Article
- Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischemia-reperfusion injuryFrontiers in cell and developmental biology · 2022Article
- A Planar Culture Model of Human Absorptive Enterocytes Reveals Metformin Increases Fatty Acid Oxidation and Export.Cellular and molecular gastroenterology and hepatology · 2022Article
- Article
- Rspo3 regulates the abnormal differentiation of small intestinal epithelial cells in diabetic state.Stem cell research & therapy · 2021Article
- Quantitative classification of chromatin dynamics reveals regulators of intestinal stem cell differentiation.Development (Cambridge, England) · 2020Article
- It's All About Timing: Early Feeding Promotes Intestinal Maturation by Shifting the Ratios of Specialized Epithelial Cells in Chicks.Frontiers in physiology · 2020Article
- Derivation of adult canine intestinal organoids for translational research in gastroenterology.BMC biology · 2019Article
- RNA regulons are essential in intestinal homeostasis.American journal of physiology. Gastrointestinal and liver physiology · 2019Review
- IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model.Cellular and molecular gastroenterology and hepatology · 2019Article
- A Monolayer of Primary Colonic Epithelium Generated on a Scaffold with a Gradient of Stiffness for Drug Transport Studies.Analytical chemistry · 2018Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The inability to identify, isolate, and culture intestinal epithelial stem cells (IESCs) has been prohibitive to the study and therapeutic utilization of these cells. Using a Sox9(EGFP) mouse model, we demonstrate that Sox9(EGFP) fluorescence signatures can be used to differentiate between and enrich for progenitors (Sox9(EGFPsubLo)) and multipotent IESCs (Sox9(EGFPlo)). Sox9(EGFPlo) cells generate "organoids" in a recently defined culture system that mimics the native IESC niche. These organoids possess all four differentiated cell types of the small intestine epithelium, demonstrating the multipotent capacity of Sox9(EGFPlo) cells. Our results are consistent with the previously reported observation that single IESCs generate cryptlike units without a detectable mesenchymal cell component. A prospective search revealed that CD24 is expressed in the Sox9(EGFPlo) population and marks IESCs that form organoids in culture. CD24 represents the first cell surface marker that facilitates fluorescence-activated cell sorting enrichment of IESCs with widely available antibodies without requiring a specialized fluorescent reporter gene mouse model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.