ArticleNucleic acids research2010
Single-molecule imaging of Bacteroides fragilis AddAB reveals the highly processive translocation of a single motor helicase.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy.Nucleic acids research · 2026Article
- Structures and single-molecule analysis of bacterial motor nuclease AdnAB illuminate the mechanism of DNA double-strand break resection.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Generation of Markerless Deletions in the Nosocomial PathogenApplied and environmental microbiology · 2019Article
- Novel large-scale chromosomal transfer in Bacteroides fragilis contributes to its pan-genome and rapid environmental adaptation.Microbial genomics · 2017Article
- Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases.Methods in enzymology · 2016Article
- The impact of DNA intercalators on DNA and DNA-processing enzymes elucidated through force-dependent binding kinetics.Nature communications · 2015Article
- Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis.The Journal of antimicrobial chemotherapy · 2014Article
- Recombination hotspots attenuate the coupled ATPase and translocase activities of an AddAB-type helicase-nuclease.Nucleic acids research · 2014Article
- On the mechanism of recombination hotspot scanning during double-stranded DNA break resection.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Direct observation of fluorescently labeled single-stranded λDNA molecules in a micro-flow channel.Journal of fluorescence · 2013Article
- Compression of the DNA substrate by a viral packaging motor is supported by removal of intercalating dye during translocation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain.Nucleic acids research · 2011Article
- Double strand break unwinding and resection by the mycobacterial helicase-nuclease AdnAB in the presence of single strand DNA-binding protein (SSB).The Journal of biological chemistry · 2010Article
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Authors and funding
4 authors.
Funding
Abstract
The AddAB helicase and nuclease complex is used for repairing double-strand DNA breaks in the many bacteria that do not possess RecBCD. Here, we show that AddAB, from the Gram-negative opportunistic pathogen Bacteroides fragilis, can rescue the ultraviolet sensitivity of an Escherichia coli recBCD mutant and that addAB is required for survival of B. fragilis following DNA damage. Using single-molecule observations we demonstrate that AddAB can translocate along DNA at up to 250 bp per second and can unwind an average of 14,000 bp, with some complexes capable of unwinding 40,000 bp. These results demonstrate the importance of processivity for facilitating encounters with recognition sequences that modify enzyme function during homologous recombination.
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