ArticleThe Biochemical journal1991
Band 3 protein degradation by calpain is enhanced in erythrocytes of old people.
Article in The Biochemical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Redefining the concept of erythrocyte senescence: is eryptosis fundamentally different from erythrocyte senescence.GeroScience · 2025Review
- Current understanding of eryptosis: mechanisms, physiological functions, role in disease, pharmacological applications, and nomenclature recommendations.Cell death & disease · 2025Review
- Erythrocytes Functionality in SARS-CoV-2 Infection: Potential Link with Alzheimer's Disease.International journal of molecular sciences · 2023Review
- Malaria-associated adhesion molecule activation facilitates the destruction of uninfected red blood cells.Blood advances · 2022Article
- How Do Red Blood Cells Die?Frontiers in physiology · 2021Review
- Manipulating Eryptosis of Human Red Blood Cells: A Novel Antimalarial Strategy?Frontiers in cellular and infection microbiology · 2018Review
- Erythrocyte Aging, Protection via Vesiculation: An Analysis Methodology via Oscillatory Flow.Frontiers in physiology · 2018Article
- Oxidative stress and caspase-mediated fragmentation of cytoplasmic domain of erythrocyte band 3 during blood storage.Blood transfusion = Trasfusione del sangue · 2012Article
- Alterations in band 3 protein and anion exchange in red blood cells of renal failure patients.Molecular and cellular biochemistry · 2005Article
- Calpastatin in erythrocytes of young and old individuals.The Biochemical journal · 1994Article
- Calpain (Ca(2+)-dependent thiol protease) in erythrocytes of young and old individuals.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Calpain from rat intestinal epithelial cells: age-dependent dynamics during cell differentiation.Molecular and cellular biochemistry · 1994Article
- Article
- Widespread activation of calcium-activated neutral proteinase (calpain) in the brain in Alzheimer disease: a potential molecular basis for neuronal degeneration.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Band 3 protein is a major erythrocyte transmembrane glycoprotein. We compared the degradation of band 3 protein by calpain I (a cytoplasmic, micromolar-Ca2(+)-requiring thiol proteinase) in the cells from old individuals (greater than 70 years old) to that in the cells from young ones (20-30 years old). In the young, little degradation of band 3 protein occurred in calpain-treated erythrocyte ghosts. In the old, significant band 3 protein degradation was found in erythrocyte ghosts treated similarly. The difference between young and old in the susceptibility of band 3 protein to calpain was retained in membrane vesicles (membranes stripped of peripheral proteins by NaOH) and in chymotrypsin-generated 60 kDa fragment (CH-60). The isolated N-terminal cytoplasmic 43 kDa fragment was degraded by calpain to a similar extent in old and in young. The separated 17 kDa membrane domain of the CH-60 and the trypsin-generated C-terminal 55 kDa membrane-spanning fragment were not degraded by calpain I in the young, nor in the old. Thus the N-terminal cytoplasmic domain is the domain degraded by calpain I. Enhanced sensitivity in the old is observed in intact band 3 protein and in CH-60, the isolated cytoplasmic domain being equally susceptible in young and old. The observed age-related enhanced sensitivity to calpain is consistent with the presence of modifications in band 3 protein and alterations in the association with the calpain-calpastatin system. Band 3 protein has several important functions, with modifications in the protein having implications for altered cell behaviour in the old individual.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.