ArticleAnalytical chemistry2010
Single molecule epigenetic analysis in a nanofluidic channel.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 114 citations in OpenAlex.
- The Emergence of Nanofluidics for Single-Biomolecule Manipulation and Sensing.Analytical chemistry · 2025Review
- Nanofluidic devices for the separation of biomolecules.Journal of chromatography. A · 2022Review
- Scanning Probe Lithography: State-of-the-Art and Future Perspectives.Micromachines · 2022Review
- Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.Advances in experimental medicine and biology · 2022Article
- Single-Molecule Techniques to Study Chromatin.Frontiers in cell and developmental biology · 2021Review
- Advances in Label-Free Detections for Nanofluidic Analytical Devices.Micromachines · 2020Review
- Molecular ring toss of circular BAC DNA using micropillar array for single-molecule studies.Biomicrofluidics · 2020Article
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- Single-Molecule Detection of DNA in a Nanochannel by High-Field Strength-Assisted Electrical Impedance Spectroscopy.Micromachines · 2019Article
- Analytical epigenetics: single-molecule optical detection of DNA and histone modifications.Current opinion in biotechnology · 2019Review
- Epigenetic Changes Associated with Early Life Experiences: Saliva, A Biospecimen for DNA Methylation Signatures.Current genomics · 2018Article
- Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement.Scientific reports · 2018Article
- Microfluidics-Based Chromosome Conformation Capture (3C) Technology for Examining Chromatin Organization with a Low Quantity of Cells.Analytical chemistry · 2018Article
- Single cell sequencing: a distinct new field.Clinical and translational medicine · 2017Review
- Technical advances in global DNA methylation analysis in human cancers.Journal of biological engineering · 2017Review
- Analysis of Individual Signaling Complexes by mMAPS, a Flow-Proteometric System.Current protocols in molecular biology · 2016Article
- Analysis of single nucleic acid molecules in micro- and nano-fluidics.Lab on a chip · 2016Review
- Characterization and directed evolution of a methyl-binding domain protein for high-sensitivity DNA methylation analysis.Protein engineering, design & selection : PEDS · 2015Article
- Triplex DNA: A new platform for polymerase chain reaction-based biosensor.Scientific reports · 2015Article
- Single molecule and single cell epigenomics.Methods (San Diego, Calif.) · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Epigenetic states are governed by DNA methylation and a host of modifications to histones bound with DNA. These states are essential for proper developmentally regulated gene expression and are perturbed in many diseases. There is great interest in identifying epigenetic mark placement genome wide and understanding how these marks vary among cell types, with changes in environment or according to health and disease status. Current epigenomic analyses employ bisulfite sequencing and chromatin immunoprecipitation, but query only one type of epigenetic mark at a time, DNA methylation, or histone modifications and often require substantial input material. To overcome these limitations, we established a method using nanofluidics and multicolor fluorescence microscopy to detect DNA and histones in individual chromatin fragments at about 10 Mbp/min. We demonstrated its utility for epigenetic analysis by identifying DNA methylation on individual molecules. This technique will provide the unprecedented opportunity for genome wide, simultaneous analysis of multiple epigenetic states on single molecules.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.