ArticleAmerican journal of translational research2010
Engineering of aligned skeletal muscle by micropatterning.
Article in American journal of translational research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 80 citations in OpenAlex.
- Manufacturing, Cell Regulation, and Coculture Strategies for Vascularization and Neural Innervation of Skeletal Muscle Tissue.Advanced healthcare materials · 2026Review
- Mechanically Spatio-Chimeric Fibrin Assembly Enables Vascular-Integrated Muscle Reconstruction for Volumetric Muscle Loss Repair.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Micro Pattern-Based 3D Cell Culture Platform: An Overview of Technologies and Applications.Exploration (Beijing, China) · 2026Review
- Shear-Induced Patterning of Decellularized Skeletal Muscle Extracellular Matrix for Enhanced Myogenesis.Advanced healthcare materials · 2025Article
- Novel stereo DIC characterisation of microneedle and hypodermic needle insertion.Frontiers in bioengineering and biotechnology · 2025Article
- Tissue Engineered 3D Constructs for Volumetric Muscle Loss.Annals of biomedical engineering · 2024Review
- Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance.International journal of molecular sciences · 2024Article
- Micropatterned fibrin scaffolds increase cardiomyocyte alignment and contractility for the fabrication of engineered myocardial tissue.Journal of biomedical materials research. Part A · 2023Article
- Article
- Porous biomaterial scaffolds for skeletal muscle tissue engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Myocyte Culture with Decellularized Skeletal Muscle Sheet with Observable Interaction with the Extracellular Matrix.Bioengineering (Basel, Switzerland) · 2022Article
- Intramuscular delivery of neural crest stem cell spheroids enhances neuromuscular regeneration after denervation injury.Stem cell research & therapy · 2022Article
- Multiscale-Engineered Muscle Constructs: PEG Hydrogel Micro-Patterning on an Electrospun PCL Mat Functionalized with Gold Nanoparticles.International journal of molecular sciences · 2021Article
- Skeletal Muscle Tissue Engineering: Biomaterials-Based Strategies for the Treatment of Volumetric Muscle Loss.Bioengineering (Basel, Switzerland) · 2020Review
- New volumetric CNT-doped gelatin-cellulose scaffolds for skeletal muscle tissue engineering.Nanoscale advances · 2020Article
- Etching anisotropic surface topography onto fibrin microthread scaffolds for guiding myoblast alignment.Journal of biomedical materials research. Part B, Applied biomaterials · 2020Article
- Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology.Scientific reports · 2018Article
- Matrix Topographical Cue-Mediated Myogenic Differentiation of Human Embryonic Stem Cell Derivatives.Polymers · 2017Article
- Study of myoblast differentiation using multi-dimensional scaffolds consisting of nano and micropatterns.Biomaterials research · 2017Article
- Anisotropic Materials for Skeletal-Muscle-Tissue Engineering.Advanced materials (Deerfield Beach, Fla.) · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Tissue engineered skeletal muscle has tremendous potential for the treatment of muscular injury or muscular dysfunction. However, in vitro methods to generate skeletal muscle with physiologically aligned myofiber structure remains limited. To develop a robust in vitro model that resembles the physiologically aligned structure of muscle fibers, we fabricated micropatterned polymer membranes of poly(dimethylsiloxane) (PDMS) with parallel microgrooves, and then examined the effect of micropatterning on myoblast cellular organization and the cell fusion process. In comparison to the myoblasts on non-patterned PDMS films, myoblasts on micropatterned PDMS films had well-organized F-actin assembly in close proximity to the direction of microgrooves, along with enhanced levels of myotube formation at early time points. The increase of cell cycle regulator p21(WAF/Cip1) and the organized interactions of N-cadherin in myoblasts on micropatterned surfaces may contribute to the enhanced formation of myotubes. Similar results of cellular alignment was observed when myoblasts were cultured on microfluidically patterned poly(2-hydroxyethyl methacrylate) (pHEMA) microgrooves, and the micropatterns were found to detach from the Petri dish over time. To apply this technology for generating aligned tissue-like muscle constructs, we developed a methodology to transfer the aligned myotubes to biodegradable collagen gels. Histological analysis revealed the persistence of aligned cellular organization in the collagen gels. Together, these results demonstrate that micropatterned PDMS or pHEMA can promote cell alignment and fusion along the direction of the microgrooves, and this platform can be utilized to transfer aligned myotubes on biodegradable hydrogels. This study highlights the importance of spatial cues in creating aligned skeletal muscle for tissue engineering and muscular regeneration applications.
Indexed as
Identifiers
20182581PMC2826821W5410578What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.