ArticleAmerican journal of physiology. Cell physiology2010
Proteomic analysis of V-ATPase-rich cells harvested from the kidney and epididymis by fluorescence-activated cell sorting.
Article in American journal of physiology. Cell physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- Transcriptome and Metabolome Analyses Reveal High-Altitude Adaptation Mechanism of Epididymis Sperm Maturation in Tibetan Sheep.Animals : an open access journal from MDPI · 2024Article
- Review
- β3-Adrenoceptor as a new player in the sympathetic regulation of the renal acid-base homeostasis.Frontiers in physiology · 2024Article
- Vasopressin V1a receptor and oxytocin receptor regulate murine sperm motility differently.Life science alliance · 2023Article
- Sperm acquire epididymis-derived proteins through epididymosomes.Human reproduction (Oxford, England) · 2022Article
- Kidney intercalated cells are phagocytic and acidify internalized uropathogenic Escherichia coli.Nature communications · 2021Article
- Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity.Journal of cell science · 2019Article
- Unravelling purinergic regulation in the epididymis: activation of V-ATPase-dependent acidification by luminal ATP and adenosine.The Journal of physiology · 2019Article
- Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function.Scientific reports · 2019Article
- Extracellular Adenosine Stimulates Vacuolar ATPase-Dependent Proton Secretion in Medullary Intercalated Cells.Journal of the American Society of Nephrology : JASN · 2018Article
- Key miRNAs and target genes played roles in the development of clear cell renal cell carcinoma.Cancer biomarkers : section A of Disease markers · 2018Article
- The expression patterns of aquaporin 9, vacuolar HHistochemistry and cell biology · 2017Article
- Mapping the H(+) (V)-ATPase interactome: identification of proteins involved in trafficking, folding, assembly and phosphorylation.Scientific reports · 2015Article
- Article
- Renal type a intercalated cells contain albumin in organelles with aldosterone-regulated abundance.PloS one · 2015Article
- Carbonic anhydrase 2 deficiency leads to increased pyelonephritis susceptibility.American journal of physiology. Renal physiology · 2014Article
- Regulation of luminal acidification by the V-ATPase.Physiology (Bethesda, Md.) · 2013Review
- Expression of the rhesus glycoproteins, ammonia transporter family members, RHCG and RHBG in male reproductive organs.Reproduction (Cambridge, England) · 2013Article
- Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis.American journal of physiology. Cell physiology · 2013Article
- Altered V-ATPase expression in renal intercalated cells isolated from B1 subunit-deficient mice by fluorescence-activated cell sorting.American journal of physiology. Renal physiology · 2013Article
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8 authors at 6 institutions in 1 country.
Funding
Abstract
Proton-transporting cells are located in several tissues where they acidify the extracellular environment. These cells express the vacuolar H(+)-ATPase (V-ATPase) B1 subunit (ATP6V1B1) in their plasma membrane. We provide here a comprehensive catalog of the proteins that are expressed in these cells, after their isolation by enzymatic digestion and fluorescence-activated cell sorting (FACS) from transgenic B1-enhanced green fluorescent protein (EGFP) mice. In these mice, type A and B intercalated cells and connecting segment cells of the kidney, and narrow and clear cells of the epididymis, which all express ATP6V1B1, also express EGFP, while all other cell types are negative. The proteome of renal and epididymal EGFP-positive (EGFP(+)) cells was identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and compared with their respective EGFP-negative (EGFP(-)) cell populations. A total of 2,297 and 1,564 proteins were detected in EGFP(+) cells from the kidney and epididymis, respectively. Out of these proteins, 202 and 178 were enriched by a factor greater than 1.5 in EGFP(+) cells compared with EGFP(-) cells, in the kidney and epididymis respectively, and included subunits of the V-ATPase (B1, a4, and A). In addition, several proteins involved in intracellular trafficking, signaling, and cytoskeletal dynamics were identified. A novel common protein that was enriched in renal and epididymal EGFP(+) cells is the progesterone receptor, which might be a potential candidate for the regulation of V-ATPase-dependent proton transport. These proteomic databases provide a framework for comprehensive future analysis of the common and distinct functions of V-ATPase-B1-expressing cells in the kidney and epididymis.
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