ArticleMolecular biology of the cell2010
h3/Acidic calponin: an actin-binding protein that controls extracellular signal-regulated kinase 1/2 activity in nonmuscle cells.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Mechanoregulation and function of calponin and transgelin.Biophysics reviews · 2024Review
- Evolution and function of calponin and transgelin.Frontiers in cell and developmental biology · 2023Review
- Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells.The Journal of cell biology · 2022Article
- Serum Calponin 3 Levels in Patients with Systemic Sclerosis: Possible Association with Skin Sclerosis and Arthralgia.Journal of clinical medicine · 2021Article
- Calponin 3 promotes invasion and drug resistance of colon cancer cells.World journal of gastrointestinal oncology · 2019Article
- Establishment of a novel experimental model for muscle-invasive bladder cancer using a dog bladder cancer organoid culture.Cancer science · 2019Article
- Calponin 3 Regulates Cell Invasion and Doxorubicin Resistance in Gastric Cancer.Gastroenterology research and practice · 2019Article
- Calponin-3 is critical for coordinated contractility of actin stress fibers.Scientific reports · 2018Article
- Mechanisms of Vascular Smooth Muscle Contraction and the Basis for Pharmacologic Treatment of Smooth Muscle Disorders.Pharmacological reviews · 2016Review
- Ixodes scapularis Tick Saliva Proteins Sequentially Secreted Every 24 h during Blood Feeding.PLoS neglected tropical diseases · 2016Article
- Article
- A Conditional Knockout Mouse Model Reveals That Calponin-3 Is Dispensable for Early B Cell Development.PloS one · 2015Article
- Reply to "Letter to the editor: 'KDAC and the regulation of nonnuclear smooth muscle protein acetylation'".American journal of physiology. Cell physiology · 2014Article
- Mechanism of calponin stabilization of cross-linked actin networks.Biophysical journal · 2014Article
- Article
- CNN3 is regulated by microRNA-1 during muscle development in pigs.International journal of biological sciences · 2014Article
- Effects of basic calponin on the flexural mechanics and stability of F-actin.Cytoskeleton (Hoboken, N.J.) · 2012Article
- How does a protein with dual mitotic spindle and extracellular matrix receptor functions affect tumor susceptibility and progression?Communicative & integrative biology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Migration of fibroblasts is important in wound healing. Here, we demonstrate a role and a mechanism for h3/acidic calponin (aCaP, CNN3) in REF52.2 cell motility, a fibroblast line rich in actin filaments. We show that the actin-binding protein h3/acidic calponin associates with stress fibers in the absence of stimulation but is targeted to the cell cortex and podosome-like structures after stimulation with a phorbol ester, phorbol-12,13-dibutyrate (PDBu). By coimmunoprecipitation and colocalization, we show that extracellular signal-regulated kinase (ERK)1/2 and protein kinase C (PKC)alpha constitutively associate with h3/acidic calponin and are cotargeted with h3/acidic calponin in the presence of PDBu. This targeting can be blocked by a PKC inhibitor but does not require phosphorylation of h3/acidic calponin at the PKC sites S175 or T184. Knockdown of h3/acidic calponin results in a loss of PDBu-mediated ERK1/2 targeting, whereas PKCalpha targeting is unaffected. Caldesmon is an actin-binding protein that regulates actomyosin interactions and is a known substrate of ERK1/2. Both ERK1/2 activity and nonmuscle l-caldesmon phosphorylation are blocked by h3/acidic calponin knockdown. Furthermore, h3/acidic calponin knockdown inhibits REF52.2 migration in an in vitro wound healing assay. Our findings are consistent with a model whereby h3/acidic calponin controls fibroblast migration by regulation of ERK1/2-mediated l-caldesmon phosphorylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.