ArticleMolecular biology of the cell2010
Deficiency of the transcriptional regulator p8 results in increased autophagy and apoptosis, and causes impaired heart function.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.
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Who cites it
33 citing papers in PubMed, 2 syntheses or guidelines pooled it, 59 citations in OpenAlex.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).Autophagy · 2016Guideline
- ScRNA-seq and bulk RNA-seq identified NUPR1 as novel biomarkers related to CD4 + T cells infiltration for abdominal aortic aneurysm.Molecular biology reports · 2024Article
- Microgravity triggers ferroptosis and accelerates senescence in the MG-63 cell model of osteoblastic cells.NPJ microgravity · 2023Article
- Insights from a Computational-Based Approach for Analyzing Autophagy Genes across Human Cancers.Genes · 2023Review
- Circulating cardiac MicroRNAs safeguard against dilated cardiomyopathy.Clinical and translational medicine · 2023Article
- Article
- The expression of apoptosis related genes in HK-2 cells overexpressingFrontiers in genetics · 2022Article
- Integrative Analyses of Genes Associated With Right Ventricular Cardiomyopathy Induced by Tricuspid Regurgitation.Frontiers in genetics · 2021Article
- The cell fate regulator NUPR1 is induced by Mycobacterium leprae via type I interferon in human leprosy.PLoS neglected tropical diseases · 2019Article
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- A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids.Redox biology · 2019Article
- Inactivation of NUPR1 promotes cell death by coupling ER-stress responses with necrosis.Scientific reports · 2018Article
- Article
- The ULK3 Kinase Is Critical for Convergent Control of Cancer-Associated Fibroblast Activation by CSL and GLI.Cell reports · 2017Article
- A liver stress-endocrine nexus promotes metabolic integrity during dietary protein dilution.The Journal of clinical investigation · 2016Article
- Gene Expression in Mouse Thyrotrope Adenoma: Transcription Elongation Factor Stimulates Proliferation.Endocrinology · 2016Article
- The transcription factor p8 regulates autophagy during diapause embryo formation in Artemia parthenogenetica.Cell stress & chaperones · 2016Article
- Transcriptome profiling of the rat retina after optic nerve transection.Scientific reports · 2016Article
- Nupr1/Chop signal axis is involved in mitochondrion-related endothelial cell apoptosis induced by methamphetamine.Cell death & disease · 2016Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Autophagy is a cytoprotective pathway used to degrade and recycle cytoplasmic content. Dysfunctional autophagy has been linked to both cancer and cardiomyopathies. Here, we show a role for the transcriptional regulator p8 in autophagy. p8 RNA interference (RNAi) increases basal autophagy markers in primary cardiomyocytes, in H9C2 and U2OS cells, and decreases cellular viability after autophagy induction. This autophagy is associated with caspase activation and is blocked by atg5 silencing and by pharmacological inhibitors. FoxO3 transcription factor was reported to activate autophagy by enhancing the expression of autophagy-related genes. P8 expression represses FoxO3 transcriptional activity, and p8 knockdown affects FoxO3 nuclear localization. Thus, p8 RNAi increases FoxO3 association with bnip3 promoter, a known proautophagic FoxO3 target, resulting in higher bnip3 RNA and protein levels. Accordingly, bnip3 knockdown restores cell viability and blocks apoptosis of p8-deficient cells. In vivo, p8 -/- mice have higher autophagy and express higher cardiac bnip3 levels. These mice develop left ventricular wall thinning and chamber dilation, with consequent impaired cardiac function. Our studies provide evidence of a p8-dependent mechanism regulating autophagy by acting as FoxO3 corepressor, which may be relevant for diseases associated with dysregulated autophagy, as cardiovascular pathologies and cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.