ArticleMolecular pharmacology2010
High-throughput multiplexed transcript analysis yields enhanced resolution of 5-hydroxytryptamine 2C receptor mRNA editing profiles.
Article in Molecular pharmacology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- Interaction of the Vagus Nerve and Serotonin in the Gut-Brain Axis.International journal of molecular sciences · 2025Review
- Regulation and functional consequences of mGluRNA (New York, N.Y.) · 2021Article
- A Comprehensive Review of Genetically Engineered Mouse Models for Prader-Willi Syndrome Research.International journal of molecular sciences · 2021Review
- International Union of Basic and Clinical Pharmacology. CX. Classification of Receptors for 5-hydroxytryptamine; Pharmacology and Function.Pharmacological reviews · 2021Review
- Quantitative Analysis of Adenosine-to-Inosine RNA Editing.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Ectopic expression of Snord115 in choroid plexus interferes with editing but not splicing of 5-Ht2c receptor pre-mRNA in mice.Scientific reports · 2019Article
- Using mouse models to unlock the secrets of non-synonymous RNA editing.Methods (San Diego, Calif.) · 2019Review
- A-to-I RNA editing in the rat brain is age-dependent, region-specific and sensitive to environmental stress across generations.BMC genomics · 2018Article
- Comparative analysis of A-to-I editing in human and non-human primate brains reveals conserved patterns and context-dependent regulation of RNA editing.Molecular brain · 2017Article
- Expanding a fluorescent RNA alphabet: synthesis, photophysics and utility of isothiazole-derived purine nucleoside surrogates.Chemical science · 2017Article
- Reduced levels of protein recoding by A-to-I RNA editing in Alzheimer's disease.RNA (New York, N.Y.) · 2016Article
- Decrease of mRNA Editing after Spinal Cord Injury is Caused by Down-regulation of ADAR2 that is Triggered by Inflammatory Response.Scientific reports · 2015Article
- A Fluorescent Adenosine Analogue as a Substrate for an A-to-I RNA Editing Enzyme.Angewandte Chemie (International ed. in English) · 2015Article
- Prevention of 5-hydroxytryptamine2C receptor RNA editing and alternate splicing in C57BL/6 mice activates the hypothalamic-pituitary-adrenal axis and alters mood.The European journal of neuroscience · 2014Article
- A unique gene expression signature associated with serotonin 2C receptor RNA editing in the prefrontal cortex and altered in suicide.Human molecular genetics · 2014Article
- Reovirus-mediated induction of ADAR1 (p150) minimally alters RNA editing patterns in discrete brain regions.Molecular and cellular neurosciences · 2014Article
- Functional status of the serotonin 5-HT2C receptor (5-HT2CR) drives interlocked phenotypes that precipitate relapse-like behaviors in cocaine dependence.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Article
- Serotonin 2c receptor RNA editing in major depression and suicide.The world journal of biological psychiatry : the official journal of the World Federation of Societies of Biological Psychiatry · 2013Article
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
RNA editing is a post-transcriptional modification in which adenosine residues are converted to inosine (adenosine-to-inosine editing). Commonly used methodologies to quantify RNA editing levels involve either direct sequencing or pyrosequencing of individual cDNA clones. The limitations of these methods lead to a small number of clones characterized in comparison to the number of mRNA molecules in the original sample, thereby producing significant sampling errors and potentially erroneous conclusions. We have developed an improved method for quantifying RNA editing patterns that increases sequence analysis to an average of more than 800,000 individual cDNAs per sample, substantially increasing accuracy and sensitivity. Our method is based on the serotonin 2C receptor (5-hydroxytryptamine(2C); 5HT(2C)) transcript, an RNA editing substrate in which up to five adenosines are modified. Using a high-throughput multiplexed transcript analysis, we were able to quantify accurately the expression of twenty 5HT(2C) isoforms, each representing at least 0.25% of the total 5HT(2C) transcripts. Furthermore, this approach allowed the detection of previously unobserved changes in 5HT(2C) editing in RNA samples isolated from different inbred mouse strains and dissected brain regions, as well as editing differences in alternatively spliced 5HT(2C) variants. This approach provides a novel and efficient strategy for large-scale analyses of RNA editing and may prove to be a valuable tool for uncovering new information regarding editing patterns in specific disease states and in response to pharmacological and physiological perturbation, further elucidating the impact of 5HT(2C) RNA editing on central nervous system function.
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Registered trials
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