ArticleEndocrinology2010
Real-time monitoring of chemical transmission in slices of the murine adrenal gland.
Article in Endocrinology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Dioxythiophene/Nafion Polymer Composite Membranes for Tunable Size-Based Selectivity in the Voltammetric Detection of Small Neuropeptides.ACS sensors · 2024Article
- Basal and Stress-Induced Network Activity in the Adrenal MedullaFrontiers in endocrinology · 2022Article
- Unbalanced Regulation of α7 nAChRs by Ly6h and NACHO Contributes to Neurotoxicity in Alzheimer's Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- 5 ns electric pulses induce CaBioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- Fast-Scan Cyclic Voltammetry: Chemical Sensing in the Brain and Beyond.Analytical chemistry · 2018Review
- Gap junction communication between chromaffin cells: the hidden face of adrenal stimulus-secretion coupling.Pflugers Archiv : European journal of physiology · 2018Review
- Enhanced Ca(2+)-induced Ca(2+) release from intracellular stores contributes to catecholamine hypersecretion in adrenal chromaffin cells from spontaneously hypertensive rats.Pflugers Archiv : European journal of physiology · 2015Article
- Electrochemical Analysis of Neurotransmitters.Annual review of analytical chemistry (Palo Alto, Calif.) · 2015Review
- Selective catecholamine recognition with NeuroSensor 521: a fluorescent sensor for the visualization of norepinephrine in fixed and live cells.ACS chemical neuroscience · 2013Article
- Stress-induced changes in adrenal neuropeptide Y expression are regulated by a negative feedback loop.Journal of neurochemistry · 2013Article
- PAP and NT5E inhibit nociceptive neurotransmission by rapidly hydrolyzing nucleotides to adenosine.Molecular pain · 2011Article
- Higher sensitivity dopamine measurements with faster-scan cyclic voltammetry.Analytical chemistry · 2011Article
- Distinguishing splanchnic nerve and chromaffin cell stimulation in mouse adrenal slices with fast-scan cyclic voltammetry.American journal of physiology. Cell physiology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The real-time electrochemical detection of catecholamine secretion from murine adrenal slices using fast-scan cyclic voltammetry (FSCV) and amperometry at carbon fiber microelectrodes is described. Bright-field and immunofluorescent microscopy supported that chromaffin cells in the adrenal medulla are organized into clusters and positively stain for tyrosine hydroxylase confirming that they are catecholaminergic. Spontaneous exocytotic catecholamine events were observed inside chromaffin cell clusters with both FSCV and amperometry and were modulated by the nicotinic acetylcholine receptor antagonist hexamethonium and low extracellular calcium. Reintroduction of extracellular calcium and pressure ejection of acetylcholine caused the frequency of spikes to increase back to predrug levels. Electrical stimulation caused the synchronous secretion from multiple cells within the gland, which were modulated by nicotinic acetylcholine receptors but not muscarinic receptors or gap junctions. Furthermore, electrically stimulated release was abolished with perfusion of low extracellular calcium or tetrodotoxin, indicating that the release requires electrical excitability. An extended waveform was used to study the spontaneous and stimulated release events to determine their chemical content by FSCV. Consistent with total content analysis and immunohistochemical studies, about two thirds of the cells studied spontaneously secreted epinephrine, whereas one third secreted norepinephrine. Whereas adrenergic sites contained mostly epinephrine during electrical stimulation, noradrenergic sites contained a mixture of the catecholamines showing the heterogeneity of the adrenal medulla.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.