ArticlePlant physiology2010
Interdependence of endomembrane trafficking and actin dynamics during polarized growth of Arabidopsis pollen tubes.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
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Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it, 95 citations in OpenAlex.
- A multiscale growth atlas of Arabidopsis: linking cell dynamics to organ development.The New phytologist · 2026Pooled it
- Establishment of fluorescent protein-tagged lines for investigating dynamic localization of organelle and other cell structures in rice pollen tube.Plant methods · 2026Article
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Rab5 GTPases mediate the targeting of ROP signalling to establish polarity for pollen germination.Nature plants · 2025Article
- Reciprocal regulation between AtFH5-labeled secretory vesicles and PI(4,5)PJournal of integrative plant biology · 2025Article
- Genome Wide Identification and Characterization of LIM Domain Associated Gene Superfamily in Common Bean (International journal of genomics · 2025Article
- Differential sensitivity of ADF isovariants to a pH gradient promotes pollen tube growth.The Journal of cell biology · 2023Article
- Low Concentration of Aluminum-Stimulated Pollen Tube Growth of Apples (Plants (Basel, Switzerland) · 2022Article
- Effect of boron toxicity on pollen tube cell wall architecture and the relationship of cell wall components ofFrontiers in plant science · 2022Article
- VPS18-regulated vesicle trafficking controls the secretion of pectin and its modifying enzyme during pollen tube growth in Arabidopsis.The Plant cell · 2021Article
- Lettuce-based production of an oral vaccine against porcine edema disease for the seed lot system.Plant biotechnology (Tokyo, Japan) · 2021Article
- Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth.The protein journal · 2021Article
- The Tip-Localized Phosphatidylserine Established by Arabidopsis ALA3 Is Crucial for Rab GTPase-Mediated Vesicle Trafficking and Pollen Tube Growth.The Plant cell · 2020Article
- Control of the Actin Cytoskeleton Within Apical and Subapical Regions of Pollen Tubes.Frontiers in cell and developmental biology · 2020Review
- The Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes.iScience · 2019Article
- Golgi-localized LOT regulatesProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Arabidopsis class I formins control membrane-originated actin polymerization at pollen tube tips.PLoS genetics · 2018Article
- Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes.Open biology · 2018Article
- The REN4 rheostat dynamically coordinates the apical and lateral domains of Arabidopsis pollen tubes.Nature communications · 2018Article
- LRX Proteins Play a Crucial Role in Pollen Grain and Pollen Tube Cell Wall Development.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During polarized growth of pollen tubes, endomembrane trafficking and actin polymerization are two critical processes that establish membrane/wall homeostasis and maintain growth polarity. Fine-tuned interactions between these two processes are therefore necessary but poorly understood. To better understand such cross talk in the model plant Arabidopsis (Arabidopsis thaliana), we first established optimized concentrations of drugs that interfere with either endomembrane trafficking or the actin cytoskeleton, then examined pollen tube growth using fluorescent protein markers that label transport vesicles, endosomes, or the actin cytoskeleton. Both brefeldin A (BFA) and wortmannin disturbed the motility and structural integrity of ARA7- but not ARA6-labeled endosomes, suggesting heterogeneity of the endosomal populations. Disrupting endomembrane trafficking by BFA or wortmannin perturbed actin polymerization at the apical region but not in the longitudinal actin cables in the shank. The interference of BFA/wortmannin with actin polymerization was progressive rather than rapid, suggesting an indirect effect, possibly due to perturbed endomembrane trafficking of certain membrane-localized signaling proteins. Both the actin depolymerization drug latrunculin B and the actin stabilization drug jasplakinolide rapidly disrupted transport of secretory vesicles, but each drug caused distinct responses on different endosomal populations labeled by ARA6 or ARA7, indicating that a dynamic actin cytoskeleton was critical for some steps in endomembrane trafficking. Our results provide evidence of cross talk between endomembrane trafficking and the actin cytoskeleton in pollen tubes.
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