ArticlePlant physiology2010
Polyphosphoinositides are enriched in plant membrane rafts and form microdomains in the plasma membrane.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 111 citations in OpenAlex.
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- Nanoscale clustering and dynamics of phosphatidylinositol 4,5-bisphosphate in an immune cell model.Biophysical journal · 2025Article
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Membrane nanodomains to shape plant cellular functions and signaling.The New phytologist · 2025Review
- Phospholipid Signaling in Crop Plants: A Field to Explore.Plants (Basel, Switzerland) · 2024Article
- The zinc finger protein DHHC09 S-acylates the kinase STRK1 to regulate H2O2 homeostasis and promote salt tolerance in rice.The Plant cell · 2024Article
- Structural determinants of REMORIN nanodomain formation in anionic membranes.Biophysical journal · 2023Article
- Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth ofPlants (Basel, Switzerland) · 2022Article
- Lipid Rafts and Plant Gravisensitivity.Life (Basel, Switzerland) · 2022Review
- Phytosterol metabolism in plant positive-strand RNA virus replication.Plant cell reports · 2022Review
- Plasma membrane phospholipid signature recruits the plant exocyst complex via the EXO70A1 subunit.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Hot topic: Thermosensing in plants.Plant, cell & environment · 2021Review
- Plasma membrane nano-organization specifies phosphoinositide effects on Rho-GTPases and actin dynamics in tobacco pollen tubes.The Plant cell · 2021Article
- Connecting the dots: from nanodomains to physiological functions of REMORINs.Plant physiology · 2021Review
- Molecular architecture of the endocytic TPLATE complex.Science advances · 2021Article
- Striking a balance: PIPExploration of neuroprotective therapy · 2021Article
- Phospholipid Signaling Is a Component of the Salicylic Acid Response in Plant Cell Suspension Cultures.International journal of molecular sciences · 2020Review
- Coordinated Localization and Antagonistic Function of NtPLC3 and PI4P 5-Kinases in the Subapical Plasma Membrane of Tobacco Pollen Tubes.Plants (Basel, Switzerland) · 2020Article
- The Nanoscale Organization of the Plasma Membrane and Its Importance in Signaling: A Proteolipid Perspective.Plant physiology · 2020Review
- Dynamics and Endocytosis of Flot1 inInternational journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this article, we analyzed the lipid composition of detergent-insoluble membranes (DIMs) purified from tobacco (Nicotiana tabacum) plasma membrane (PM), focusing on polyphosphoinositides, lipids known to be involved in various signal transduction events. Polyphosphoinositides were enriched in DIMs compared with whole PM, whereas all structural phospholipids were largely depleted from this fraction. Fatty acid composition analyses suggest that enrichment of polyphosphoinositides in DIMs is accompanied by their association with more saturated fatty acids. Using an immunogold-electron microscopy strategy, we were able to visualize domains of phosphatidylinositol 4,5-bisphosphate in the plane of the PM, with 60% of the epitope found in clusters of approximately 25 nm in diameter and 40% randomly distributed at the surface of the PM. Interestingly, the phosphatidylinositol 4,5-bisphosphate cluster formation was not significantly sensitive to sterol depletion induced by methyl-beta-cyclodextrin. Finally, we measured the activities of various enzymes of polyphosphoinositide metabolism in DIMs and PM and showed that these activities are present in the DIM fraction but not enriched. The putative role of plant membrane rafts as signaling membrane domains or membrane-docking platforms is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.