ArticlePlant physiology2010
Cloning of the papaya chromoplast-specific lycopene beta-cyclase, CpCYC-b, controlling fruit flesh color reveals conserved microsynteny and a recombination hot spot.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 102 citations in OpenAlex.
- Advances in engineering the production of the natural red pigment lycopene: A systematic review from a biotechnology perspective.Journal of advanced research · 2023Pooled it
- Genome-Wide Association Analysis of Sweet Pepper (Plants (Basel, Switzerland) · 2025Article
- Unraveling the Genetic Control of Pigment Accumulation in Physalis Fruits.International journal of molecular sciences · 2024Article
- Article
- A point mutation in the zinc-finger transcription factorFrontiers in plant science · 2024Article
- Quality trait improvement in horticultural crops: OMICS and modern biotechnological approaches.Molecular biology reports · 2023Review
- PutativePlants (Basel, Switzerland) · 2023Article
- Marker-Assisted Selection in Breeding for Fruit Trait Improvement: A Review.International journal of molecular sciences · 2023Review
- Molecular Cloning and Functional Analysis ofFoods (Basel, Switzerland) · 2022Article
- Identification of QTLs for root color and carotenoid contents in Japanese orange carrot FScientific reports · 2022Article
- A comprehensive analysis of carotenoids metabolism in two red-fleshed mutants of Navel and Valencia sweet oranges (Frontiers in plant science · 2022Article
- Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content.Genes · 2021Review
- A single amino acid insertion in LCYB2 deflects carotenoid biosynthesis in red carrot.Plant cell reports · 2021Article
- Discovery of SNPs and InDels in papaya genotypes and its potential for marker assisted selection of fruit quality traits.Scientific reports · 2021Article
- Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit.Journal of experimental botany · 2020Article
- Genomic variation between PRSV resistant transgenic SunUp and its progenitor cultivar Sunset.BMC genomics · 2020Article
- Transcriptome Profiling ofPlants (Basel, Switzerland) · 2020Article
- An ultra-high-density genetic map provides insights into genome synteny, recombination landscape and taproot skin colour in radish (Raphanus sativus L.).Plant biotechnology journal · 2020Article
- Expression of a Chromoplast-Specific Lycopene β-Cyclase Gene (Biomolecules · 2019Article
- Exploring the differential mechanisms of carotenoid biosynthesis in the yellow peel and red flesh of papaya.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carotenoid pigments in fruits are indicative of the ripening process and potential nutritional value. Papaya (Carica papaya) fruit flesh color is caused by the accumulation of lycopene or beta-carotenoids in chromoplasts. It is a distinct feature affecting nutritional composition, fruit quality, shelf life, and consumer preference. To uncover the molecular basis of papaya flesh color, we took map-based cloning and candidate gene approaches using integrated genetic and physical maps. A DNA marker tightly linked to flesh color colocalized on a contig of the physical map with a cDNA probe of the tomato (Solanum lycopersicum) chromoplast-specific lycopene beta-cyclase, CYC-b. Candidate gene sequences were obtained from amplified fragments and verified by sequencing two bacterial artificial chromosomes containing the two alleles. Sequence comparison revealed a 2-bp insertion in the coding region of the recessive red flesh allele resulting in a frame-shift mutation and a premature stop codon. A color complementation test in bacteria confirmed that the papaya CpCYC-b is the gene controlling fruit flesh color. Sequence analysis of wild and cultivated papaya accessions showed the presence of this frame-shift mutation in all red flesh accessions examined. Evaluation of DNA markers near CpCYC-b revealed a recombination hot spot, showing that CpCYC-b is located in a gene-rich region with a recombination rate at 3.7 kb per centimorgan, more than 100-fold higher than the genome average at 400 kb per centimorgan. Conserved microsynteny of the CpCYC-b region is indicated by colinearity of two to four genes between papaya, Arabidopsis (Arabidopsis thaliana), grape (Vitis vinifera), and tomato. Our results enhanced our understanding of papaya flesh color inheritance and generated new tools for papaya improvement.
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