ArticleRNA (New York, N.Y.)2010
Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Nucleic acid-protein interfaces studied by MAS solid-state NMR spectroscopy.Journal of structural biology: X · 2022Article
- Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA.Nature communications · 2018Article
- Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly.Nucleic acids research · 2016Article
- Protein-RNA specificity by high-throughput principal component analysis of NMR spectra.Nucleic acids research · 2015Article
- Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5.Biochemistry · 2014Article
- Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution.Nature structural & molecular biology · 2014Article
- It takes two to tangle: Prp24 and spliceosome assembly.Nature structural & molecular biology · 2014Article
- Crystal structures of the Lsm complex bound to the 3' end sequence of U6 small nuclear RNA.Nature · 2014Article
- The spliceosome: disorder and dynamics defined.Current opinion in structural biology · 2014Review
- Structural analyses of the pre-mRNA splicing machinery.Protein science : a publication of the Protein Society · 2013Review
- Solution structure of the two RNA recognition motifs of hnRNP A1 using segmental isotope labeling: how the relative orientation between RRMs influences the nucleic acid binding topology.Journal of biomolecular NMR · 2013Article
- Three RNA recognition motifs participate in RNA recognition and structural organization by the pro-apoptotic factor TIA-1.Journal of molecular biology · 2012Article
- Modular protein-RNA interactions regulating mRNA metabolism: a role for NMR.European biophysics journal : EBJ · 2011Review
- A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop.Nucleic acids research · 2011Article
- Structural basis for the dual RNA-recognition modes of human Tra2-β RRM.Nucleic acids research · 2011Article
- Crystal structure of the RNA recognition motif of yeast translation initiation factor eIF3b reveals differences to human eIF3b.PloS one · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
U6 RNA plays a critical role in pre-mRNA splicing. Assembly of U6 into the spliceosome requires a significant structural rearrangement and base-pairing with U4 RNA. In the yeast Saccharomyces cerevisiae, this process requires the essential splicing factor Prp24. We present the characterization and structure of a complex containing one of Prp24's four RNA recognition motif (RRM) domains, RRM2, and a fragment of U6 RNA. NMR methods were used to identify the preferred U6 binding sequence of RRM2 (5'-GAGA-3'), measure the affinity of the interaction, and solve the structure of RRM2 bound to the hexaribonucleotide AGAGAU. Interdomain contacts observed between RRM2 and RRM3 in a crystal structure of the free protein are not detectable in solution. A structural model of RRM1 and RRM2 bound to a longer segment of U6 RNA is presented, and a partial mechanism for Prp24's annealing activity is proposed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.