ArticleJournal of virology2010
The length of and nonhydrophobic residues in the transmembrane domain of dengue virus envelope protein are critical for its retention and assembly in the endoplasmic reticulum.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Pseudovirus as an Emerging Reference Material in Molecular Diagnostics: Advancement and Perspective.Current issues in molecular biology · 2025Review
- Spatiotemporal dispersion of DENV-1 genotype V in Western Colombia.Virus evolution · 2025Article
- Dual EMCV-IRES-integrated dengue virus can express an exogenous gene and cellular Mdm2 integration suppresses the dengue viral replication.Frontiers in microbiology · 2025Article
- Characterization of Membrane Topology and Retention Signal of Pestiviral Glycoprotein E1.Journal of virology · 2021Article
- The key amino acids of E protein involved in early flavivirus infection: viral entry.Virology journal · 2021Review
- Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection.Archives of virology · 2021Article
- Structure-guided paradigm shifts in flavivirus assembly and maturation mechanisms.Advances in virus research · 2020Review
- Infection of Aedes albopictus Mosquito C6/36 Cells with theJournal of virology · 2019Article
- Role of Capsid Anchor in the Morphogenesis of Zika Virus.Journal of virology · 2018Article
- Human antibody responses after dengue virus infection are highly cross-reactive to Zika virus.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment.Virology · 2014Article
- Induction of endoplasmic reticulum-derived replication-competent membrane structures by West Nile virus non-structural protein 4B.PloS one · 2014Article
- Anchors aweigh: protein localization and transport mediated by transmembrane domains.Trends in cell biology · 2013Review
- Sulfated polysaccharide, curdlan sulfate, efficiently prevents entry/fusion and restricts antibody-dependent enhancement of dengue virus infection in vitro: a possible candidate for clinical application.PLoS neglected tropical diseases · 2013Article
- Review
- Article
- The major determinant of attenuation in mice of the Candid1 vaccine for Argentine hemorrhagic fever is located in the G2 glycoprotein transmembrane domain.Journal of virology · 2011Article
- Static retention of the lumenal monotopic membrane protein torsinA in the endoplasmic reticulum.The EMBO journal · 2011Article
- Vaccination with dengue virus-like particles induces humoral and cellular immune responses in mice.Virology journal · 2011Article
- The unique transmembrane hairpin of flavivirus fusion protein E is essential for membrane fusion.Journal of virology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The morphogenesis of many enveloped viruses, in which viral nucleocapsid complex interacts with envelope (E) protein, is known to take place at various sites along the secretory pathway. How viral E protein retains in a particular intracellular organelle for assembly remains incompletely understood. In this study, we investigated determinants in the E protein of dengue virus (DENV) for its retention and assembly in the endoplasmic reticulum (ER). A chimeric experiment between CD4 and DENV precursor membrane/E constructs suggested that the transmembrane domain (TMD) of E protein contains an ER retention signal. Substitutions of three nonhydrophobic residues at the N terminus of the first helix (T1) and at either the N or C terminus of the second helix of the TMD with three hydrophobic residues, as well as an increase in the length of T1, led to the release of chimeric CD4 and E protein from the ER, suggesting that short length and certain nonhydrophobic residues of the TMD are critical for ER retention. The analysis of enveloped viruses assembled at the plasma membrane and of those assembled in the Golgi complex and ER revealed a trend of decreasing length and increasing nonhydrophobic residues of the TMD of E proteins. Taken together, these findings support a TMD-dependent sorting for viral E proteins along the secretory pathway. Moreover, similar mutations introduced into the TMD of DENV E protein resulted in the increased production of virus-like particles (VLPs), suggesting that modifications of TMD facilitate VLP production and have implications for utilizing flaviviral VLPs as serodiagnostic antigens and vaccine candidates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.