ArticlePlant methods2010
A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine.
Article in Plant methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed, 210 citations in OpenAlex.
- EdU-Based Quantitative Analysis of Cell Proliferation in Arabidopsis Root Meristems.Methods in molecular biology (Clifton, N.J.) · 2027Article
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- The Arabidopsis ARF3-AIP1/2-SAP18 module specifies the root stem cell niche in response to auxin.The Plant cell · 2026Article
- Auxin transport positions stem cells in the vascular cambium during normal development and regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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- ESSENTIAL MEIOTIC ENDONUCLEASE 1 is required for chloroplast development and DNA repair in rice.Plant biotechnology journal · 2025Article
- A comparison of genomic methods to assess DNA replication timing.Scientific reports · 2025Article
- A Method to Visualize Cell Proliferation ofPlant direct · 2025Article
- Ethylene-independent modulation of root development by ACC via downregulation of WOX5 and group I CLE peptide expression.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Establishing of 3D-FISH on frozen section and its applying in chromosome territories analysis in Populus trichocarpa.Plant cell reports · 2024Article
- CELLULOSE SYNTHASE-LIKE C proteins modulate cell wall establishment during ethylene-mediated root growth inhibition in rice.The Plant cell · 2024Article
- A conserved GRAS-domain transcriptional regulator links meristem indeterminacy to sex determination in Ceratopteris gametophytes.Current biology : CB · 2024Article
- ARGONAUTE10 controls cell fate specification and formative cell divisions in the Arabidopsis root.The EMBO journal · 2024Article
- H2A.X promotes endosperm-specific DNA methylation in Arabidopsis thaliana.BMC plant biology · 2023Article
- Plastid-localized amino acid metabolism coordinates rice ammonium tolerance and nitrogen use efficiency.Nature plants · 2023Article
- A lignin-derived material improves plant nutrient bioavailability and growth through its metal chelating capacity.Nature communications · 2023Article
- MEDIATOR SUBUNIT17 is required for transcriptional optimization of root system architecture in Arabidopsis.Plant physiology · 2023Article
- Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium.Nature plants · 2023Article
- SNF1-related protein kinase 1 represses Arabidopsis growth through post-translational modification of E2Fa in response to energy stress.The New phytologist · 2023Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProgress in plant cell cycle research is highly dependent on reliable methods for detection of cells replicating DNA. Frequency of S-phase cells (cells in DNA synthesis phase) is a basic parameter in studies on the control of cell division cycle and the developmental events of plant cells. Here we extend the microscopy and flow cytometry applications of the recently developed EdU (5-ethynyl-2'-deoxyuridine)-based S-phase assay to various plant species and tissues. We demonstrate that the presented protocols insure the improved preservation of cell and tissue structure and allow significant reduction in assay duration. In comparison with the frequently used detection of bromodeoxyuridine (BrdU) and tritiated-thymidine incorporation, this new methodology offers several advantages as we discuss here.
resultsApplications of EdU-based S-phase assay in microscopy and flow cytometry are presented by using cultured cells of alfalfa, Arabidopsis, grape, maize, rice and tobacco. We present the advantages of EdU assay as compared to BrdU-based replication assay and demonstrate that EdU assay -which does not require plant cell wall digestion or DNA denaturation steps, offers reduced assay duration and better preservation of cellular, nuclear and chromosomal morphologies. We have also shown that fast and efficient EdU assay can also be an efficient tool for dual parameter flow cytometry analysis and for quantitative assessment of replication in thick root samples of rice.
conclusionsIn plant cell cycle studies, EdU-based S-phase detection offers a superior alternative to the existing S-phase assays. EdU method is reliable, versatile, fast, simple and non-radioactive and it can be readily applied to many different plant systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.