ArticleAnalytical chemistry2010
13C-formylation for improved nuclear magnetic resonance profiling of amino metabolites in biofluids.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- NMR-based isotope editing, chemoselection and isotopomer distribution analysis in stable isotope resolved metabolomics.Methods (San Diego, Calif.) · 2022Review
- Article
- Can NMR solve some significant challenges in metabolomics?Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Review
- Multidimensional approaches to NMR-based metabolomics.Analytical chemistry · 2014Review
- 15N-cholamine--a smart isotope tag for combining NMR- and MS-based metabolite profiling.Analytical chemistry · 2013Article
- "Add to subtract": a simple method to remove complex background signals from the 1H nuclear magnetic resonance spectra of mixtures.Analytical chemistry · 2012Article
- Isotope enhanced approaches in metabolomics.Advances in experimental medicine and biology · 2012Review
- Ratio analysis nuclear magnetic resonance spectroscopy for selective metabolite identification in complex samples.Analytical chemistry · 2011Article
- Quantitative analysis of blood plasma metabolites using isotope enhanced NMR methods.Analytical chemistry · 2010Article
- Advances in NMR-based biofluid analysis and metabolite profiling.The Analyst · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
An increased interest in metabolite profiling is driving the need for improved analytical techniques with greater performance for a variety of important applications. Despite their limited sensitivity, nuclear magnetic resonance (NMR) methods are attractive because of their simplicity, reproducibility, quantitative nature, and wide applicability. The use of chemoselective isotopic tags has the potential to advance the application of NMR for analyzing metabolites in complex biofluids by allowing detection of metabolites down to the low micromoalr level with high resolution and specificity. Here, we report a new (13)C-tagging method using (13)C-formic acid that delivers high sensitivity, good quantitation, and excellent resolution for (1)H-(13)C 2D NMR profiling of amino metabolites. High reproducibility (coefficient of variation (CV) = 2%) was observed for metabolites in urine with concentrations down to 10 microM. As amino compounds comprise an important class of metabolites and small molecules of biological roles, this new method therefore should be amenable to a variety of applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.