ArticleStem cell reviews and reports2011
Convenient and efficient enrichment of the CD133+ liver cells from rat fetal liver cells as a source of liver stem/progenitor cells.
Article in Stem cell reviews and reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Differentiation-inducing therapeutic effect of Notch inhibition in reversing malignant transformation of liver normal stem cells via MET.Oncotarget · 2018Article
- The C-terminus domain of the hepatitis B virus x protein stimulates the proliferation of mouse foetal hepatic progenitor cells, although it is not required for the formation of spheroids.International journal of molecular medicine · 2017Article
- Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor.Scientific reports · 2016Article
- [EPCAM-positive normal hepatic progenitor cells transformation into liver stem cells and HBx-mediated effects on stability in adult mouse].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2015Article
- Keratinocyte growth factor and thiazolidinediones and linolenic acid differentiate characterized mammary fat pad adipose stem cells isolated from prepubertal Korean black goat to epithelial and adipogenic lineage.In vitro cellular & developmental biology. Animal · 2014Article
- Unbalanced distribution of materials: the art of giving rise to hepatocytes from liver stem/progenitor cells.Journal of cellular and molecular medicine · 2014Review
- Stages based molecular mechanisms for generating cholangiocytes from liver stem/progenitor cells.World journal of gastroenterology · 2013Review
- Human parenchymal and non-parenchymal liver cell isolation, culture and characterization.Hepatology international · 2013Article
- Interpretation of interlocking key issues of cancer stem cells in malignant solid tumors.Cellular oncology (Dordrecht, Netherlands) · 2012Review
- Expression of the stem cell markers CD133 and nestin in pancreatic ductal adenocarcinoma and clinical relevance.International journal of clinical and experimental pathology · 2012Article
- Several important in vitro improvements in the amplification, differentiation and tracing of fetal liver stem/progenitor cells.PloS one · 2012Article
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the stem cells are commonly isolated by FACS or MACS, they are very expensive and these is no specific marker for liver stem/progentior cells (LSPCs). This paper applied a convenient and efficient method to enrich LSPCs. The fetal liver cells (FLCs) were firstly enriched by Percoll discontinuous gradient centrifugation (PDGC) from the rat fetal liver. Then the FLCs in culture were purified to be homogeneous in size by differential trypsinization and differential adherence (DTDA). Flow cytometric analysis revealed more than half of the purified FLCs expressed alternative markers of LSPCs (CD117, c-Met, Sca-1, CD90, CD49f and CD133). In other words, the purified FLCs were heterogeneous. Therefore, they were sequentially layered into six fractions by Percoll continuous gradient centrifugation (PCGC). Both CD133 and CD49f expressed decreasingly from fraction 1 to 6. In fraction 1 and 2, about 85% FLCs expressed CD133, which were revealed to be LSPCs by high expressions of AFP and CK-19, low expressions of G-6-P and ALB. To conclude, the purity of CD133(+) LSPCs enriched by combination of PDGC, DTDA and PCGC is close to that obtained by MACS. This study will greatly contribute to two important biological aspects: liver stem cells isolation and liver cell therapy.
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