ArticlePlant cell reports2010
Genetic transformation and pyramiding of aprotinin-expressing sugarcane with cry1Ab for shoot borer (Chilo infuscatellus) resistance.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Establishment of an efficientFrontiers in plant science · 2026Article
- Ectopic expression of choline oxidase (3 Biotech · 2024Article
- Review
- Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- Transgenic sugarcane with higher levels of BRK1 showed improved drought tolerance.Plant cell reports · 2023Article
- Genetic engineering: an efficient approach to mitigating biotic and abiotic stresses in sugarcane cultivation.Plant signaling & behavior · 2022Article
- Genetic Engineering Approaches for Enhanced Insect Pest Resistance in Sugarcane.Molecular biotechnology · 2021Review
- hRNAi-mediated knock-down of Sphenophorus levis V-ATPase E in transgenic sugarcane (Saccharum spp interspecific hybrid) affects the insect growth and survival.Plant cell reports · 2021Article
- ImprovementGM crops & food · 2021Article
- Genetic Transformation of Sugarcane, Current Status and Future Prospects.Frontiers in plant science · 2021Review
- Ectopic expression of DJ-1/PfpI domain containing Erianthus arundinaceus Glyoxalase III (EaGly III) enhances drought tolerance in sugarcane.Plant cell reports · 2020Article
- Resistance to Chilo infuscatellus (Lepidoptera: Pyraloidea) in transgenic lines of sugarcane expressing Bacillus thuringiensis derived Vip3A protein.Molecular biology reports · 2020Article
- TinkeringInternational journal of molecular sciences · 2020Article
- Particle Bombardment of the cry2A Gene Cassette Induces Stem Borer Resistance in Sugarcane.International journal of molecular sciences · 2018Article
- Coordinated protein co-expression in plants by harnessing the synergy between an intein and a viral 2A peptide.Plant biotechnology journal · 2017Article
- Advances in Agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops.Protoplasma · 2016Review
- Vacuolar targeting of r-proteins in sugarcane leads to higher levels of purifiable commercially equivalent recombinant proteins in cane juice.Plant biotechnology journal · 2016Article
- A novel PR10 promoter from Erianthus arundinaceus directs high constitutive transgene expression and is enhanced upon wounding in heterologous plant systems.Molecular biology reports · 2016Article
- Transgenic Sugarcane with a cry1Ac Gene Exhibited Better Phenotypic Traits and Enhanced Resistance against Sugarcane Borer.PloS one · 2016Article
- Truncated Ubiquitin 5' Regulatory Region from Erianthus arundinaceus Drives Enhanced Transgene Expression in Heterologous Systems.Molecular biotechnology · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated the insecticidal toxicity of Cry1Aa, Cry1Ab and Cry1Ac toxins against neonate larvae of sugarcane shoot borer Chilo infuscatellus Snellen (Lepidoptera: Crambidae) in vitro on diet surface. With the lowest LC(50) value, Cry1Ab emerged as the most effective among the three toxins. Sugarcane cultivars Co 86032 and CoJ 64 were transformed with cry1Ab gene driven by maize ubiquitin promoter through particle bombardment and Agrobacterium-mediated transformation systems. Gene pyramiding was also attempted by retransforming sugarcane plants carrying bovine pancreatic trypsin inhibitor (aprotinin) gene, with cry1Ab. Southern analysis confirmed multiple integration of the transgene in case of particle bombardment and single site integration in Agrobacterium-mediated transformants. The expression of cry1Ab was demonstrated through Western analysis and the toxin was quantified using ELISA. The amount of Cry1Ab protein in different events varied from 0.007 to 1.73% of the total soluble leaf protein; the events transformed by Agrobacterium method showed significantly higher values. In in vivo bioassay with neonate larvae of shoot borer, transgenics produced considerably lower percentage of deadhearts despite suffering feeding damage by the borer compared with the untransformed control plants. Expressed Cry1Ab content was negatively related to deadheart damage. Aprotinin-expressing sugarcane pyramided with cry1Ab also showed reduction in damage. The potential of producing sugarcane transgenics with cry1Ab and aprotinin genes resistant to early shoot borer was discussed in the light of the results obtained.
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