ArticleMolecular therapy : the journal of the American Society of Gene Therapy2010
Comparison of AAV serotypes for gene delivery to dorsal root ganglion neurons.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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Who cites it
110 citing papers in PubMed, 191 citations in OpenAlex.
- Protocol for targeted peripheral adeno-associated viral vector transduction of the intradental neurons in mice.STAR protocols · 2026Article
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- Histone lactylation regulates DOCK4 to control heat nociception and supports Dynein-mediated Nav1.7 trafficking.Nature communications · 2025Article
- Nociceptor Neurons Facilitate Orthodontic Tooth Movement via Piezo2 in Mice.Journal of dental research · 2025Article
- Labeling phrenic afferents with intrapleural AAV-PHP.S.Journal of neuroscience methods · 2025Article
- Application of Gene Therapy to Oral Diseases.Pharmaceutics · 2025Review
- AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain.Nature communications · 2025Article
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- Effects of chemogenetic virus injection and clozapine administration in spinal cord injury.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025Article
- Comparative Analysis of Six Adeno-Associated Viral Vector Serotypes in Mouse Inferior Colliculus and Cerebellum.eNeuro · 2024Article
- Comparative analysis of six adeno-associated viral vector serotypes in mouse inferior colliculus and cerebellum.bioRxiv : the preprint server for biology · 2024Article
- Schwann cell-derived extracellular vesicles promote memory impairment associated with chronic neuropathic pain.Journal of neuroinflammation · 2024Article
- In-Vivo Calcium Imaging of Sensory Neurons in the Rat Trigeminal Ganglion.Journal of visualized experiments : JoVE · 2024Article
- Gene therapy of adeno-associated virus (AAV) vectors in preclinical models of ischemic stroke.CNS neuroscience & therapeutics · 2023Review
- Gene replacement therapy in two Golgi-retained CMT1X mutants before and after the onset of demyelinating neuropathy.Molecular therapy. Methods & clinical development · 2023Article
- Knockdown of polypyrimidine tract binding protein facilitates motor function recovery after spinal cord injury.Neural regeneration research · 2023Article
- Comparison of the efficiencies of intrathecal and intraganglionic injections in mouse dorsal root ganglion.Turkish journal of medical sciences · 2023Article
- Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism.Nature biomedical engineering · 2022Article
- Circulating neurofilament light chain as a promising biomarker of AAV-induced dorsal root ganglia toxicity in nonclinical toxicology species.Molecular therapy. Methods & clinical development · 2022Article
50 more citing papers are in PubMed but not listed here.
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9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For many experiments in the study of the peripheral nervous system, it would be useful to genetically manipulate primary sensory neurons. We have compared vectors based on adeno-associated virus (AAV) serotypes 1, 2, 3, 4, 5, 6, and 8, and lentivirus (LV), all expressing green fluorescent protein (GFP), for efficiency of transduction of sensory neurons, expression level, cellular tropism, and persistence of transgene expression following direct injection into the dorsal root ganglia (DRG), using histological quantification and qPCR. Two weeks after injection, AAV1, AAV5, and AAV6 had transduced the most neurons. The time course of GFP expression from these three vectors was studied from 1 to 12 weeks after injection. AAV5 was the most effective serotype overall, followed by AAV1. Both these serotypes showed increasing neuronal transduction rates at later time points, with some injections of AAV5 yielding over 90% of DRG neurons GFP(+) at 12 weeks. AAV6 performed well initially, but transduction rates declined dramatically between 4 and 12 weeks. AAV1 and AAV5 both transduced large-diameter neurons, IB4(+) neurons, and CGRP(+) neurons. In conclusion, AAV5 is a highly effective gene therapy vector for primary sensory neurons following direct injection into the DRG.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.