ArticleBMC biotechnology2010
PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome.
Article in BMC biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Genetic improvement of clubroot resistance in the recessive genic male sterile line YA001 inFrontiers in plant science · 2025Article
- An integrase toolbox to record gene-expression during plant development.Nature communications · 2023Article
- Design of a nanobiosystem with remote photothermal gene silencing in Chlamydomonas reinhardtii to increase lipid accumulation and production.Microbial cell factories · 2023Article
- phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans.Genetics · 2022Article
- Genetic switches designed for eukaryotic cells and controlled by serine integrases.Communications biology · 2020Article
- Phenotypic Examination ofPlants (Basel, Switzerland) · 2020Article
- A memory switch for plant synthetic biology based on the phage ϕC31 integration system.Nucleic acids research · 2020Article
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane.Plant molecular biology · 2019Article
- Small serine recombination systems ParA-MRS and CinH-RS2 perform precise excision of plastid DNA.Plant biotechnology journal · 2017Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.Development (Cambridge, England) · 2013Article
- Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).Journal of industrial microbiology & biotechnology · 2012Review
- The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis.BMC biotechnology · 2012Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe large serine recombinase phiC31 from broad host range Streptomyces temperate phage, catalyzes the site-specific recombination of two recognition sites that differ in sequence, typically known as attachment sites attB and attP. Previously, we characterized the phiC31 catalytic activity and modes of action in the fission yeast Schizosaccharomyces pombe.
resultsIn this work, the phiC31 recombinase gene was placed under the control of the Arabidopsis OXS3 promoter and introduced into Arabidopsis harboring a chromosomally integrated attB and attP-flanked target sequence. The phiC31 recombinase excised the attB and attP-flanked DNA, and the excision event was detected in subsequent generations in the absence of the phiC31 gene, indicating germinal transmission was possible. We further verified that the genomic excision was conservative and that introduction of a functional recombinase can be achieved through secondary transformation as well as manual crossing.
conclusionThe phiC31 system performs site-specific recombination in germinal tissue, a prerequisite for generating stable lines with unwanted DNA removed. The precise site-specific deletion by phiC31 in planta demonstrates that the recombinase can be used to remove selectable markers or other introduced transgenes that are no longer desired and therefore can be a useful tool for genome engineering in plants.
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