ArticleIn vitro cellular & developmental biology. Animal2010
Derivation of new human embryonic stem cell lines from preimplantation genetic screening and diagnosis-analyzed embryos.
Article in In vitro cellular & developmental biology. Animal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Female sex bias in human embryonic stem cell lines.Stem cells and development · 2012Pooled it
- Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells.The Journal of reproduction and development · 2016Article
- Sourcing human embryos for embryonic stem cell lines: problems & perspectives.The Indian journal of medical research · 2014Review
- Modeling neurodevelopmental disorders using human pluripotent stem cells.Stem cell reviews and reports · 2014Review
- Current state of the opportunities for derivation of germ-like cells from pluripotent stem cells: are you a man, or a mouse?Biotechnology, biotechnological equipment · 2014Article
- How can ethics relate to science? The case of stem cell research.European journal of human genetics : EJHG · 2013Article
- Vitrified blastocysts from Preimplantation Genetic Diagnosis (PGD) as a source for human Embryonic Stem Cell (hESC) derivation.Journal of assisted reproduction and genetics · 2012Article
- Stem cell and tissue engineering research in the Islamic republic of Iran.Stem cell reviews and reports · 2012Review
- Stimulating progress in regenerative medicine: improving the cloning and recovery of cryopreserved human pluripotent stem cells with ROCK inhibitors.Regenerative medicine · 2010Review
- The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells.International journal of fertility & sterilityArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we focused on the derivation of human embryonic stem cell (hESC) from preimplantation genetic screening (PGS)-analyzed and preimplantation genetic diagnosis (PGD)-analyzed embryos. Out of 62 fresh PGD/PGS-analyzed embryos, 22 embryos reached the blastocyst stage. From 12 outgrowth blastocysts, we derived four hESC lines onto a feeder layer. Surprisingly, karyotype analysis showed that hESC lines derived from aneuploid embryos had diploid female karyotype. One hESC line was found to carry a balanced Robertsonian translocation. All the cell lines showed hESC markers and had the pluripotent ability to differentiate into derivatives of the three embryonic germ layers. The established lines had clonal propagation with 22-31% efficiency in the presence of ROCK inhibitor. These results further indicate that hESC lines can be derived from PGD/PGS-analyzed embryos that are destined to be discarded and can serve as an alternative source for normal euploid lines.
Indexed as
Identifiers
20177993What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.