ArticlePlanta2010
Evaluation of chloroform/methanol extraction to facilitate the study of membrane proteins of non-model plants.
Article in Planta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 35 citations in OpenAlex.
- Systematic Comparison of Bone Proteome Extraction Methods to Allow for Integrated Proteomics-Metabolomics Correlation.Journal of proteome research · 2025Article
- The microbiologist's guide to metaproteomics.iMeta · 2025Review
- Mass Spectrometry Spatial-Omics on a Single Conductive Slide.Analytical chemistry · 2021Article
- Gestational Age-Dependent Abundance of Human Placental Transporters as Determined by Quantitative Targeted Proteomics.Drug metabolism and disposition: the biological fate of chemicals · 2020Article
- A New Approach of Extraction of α-Amylase/trypsin Inhibitors from Wheat (Molecules (Basel, Switzerland) · 2019Article
- Quantitative proteomic analysis of murine white adipose tissue for peritoneal cancer metastasis.Analytical and bioanalytical chemistry · 2018Article
- Optimized approaches for quantification of drug transporters in tissues and cells by MRM proteomics.The AAPS journal · 2014Review
- Proteomic analysis reveals the diversity and complexity of membrane proteins in chickpea (Cicer arietinum L.).Proteome science · 2012Article
- Integration of deep transcriptome and proteome analyses reveals the components of alkaloid metabolism in opium poppy cell cultures.BMC plant biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane proteins are of great interest to plant physiologists because of their important function in many physiological processes. However, their study is hampered by their low abundance and poor solubility in aqueous buffers. Proteomics studies of non-model plants are generally restricted to gel-based methods. Unfortunately, all gel-based techniques for membrane proteomics lack resolving power. Therefore, a very stringent enrichment method is needed before protein separation. In this study, protein extraction in a mixture of chloroform and methanol in combination with gel electrophoresis is evaluated as a method to study membrane proteins in non-model plants. Benefits as well as disadvantages of the method are discussed. To demonstrate the pitfalls of working with non-model plants and to give a proof of principle, the method was first applied to whole leaves of the model plant Arabidopsis. Subsequently, a comparison with proteins extracted from leaves of the non-model plant, banana, was made. To estimate the tissue and organelle specificity of the method, it was also applied on banana meristems. Abundant membrane or lipid-associated proteins could be identified in both tissues, with the leaf extract yielding a higher number of membrane proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.