ArticleExperimental & molecular medicine2010
Co-treatment with hepatocyte growth factor and TGF-beta1 enhances migration of HaCaT cells through NADPH oxidase-dependent ROS generation.
Article in Experimental & molecular medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- From Defense to Disease: NADPH Oxidase in Cellular Function and Dysregulation.Journal of immunology research · 2026Review
- Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells.PLoS pathogens · 2022Article
- The Overactivation of NADPH Oxidase duringAntioxidants (Basel, Switzerland) · 2021Article
- Isoegomaketone fromEvidence-based complementary and alternative medicine : eCAM · 2021Article
- NOX2 Expression Is Increased in Keratinocytes After Burn Injury.Journal of burn care & research : official publication of the American Burn Association · 2020Article
- Retracted Article: MiR-132 enhances proliferation and migration of HaCaT cells by targeting TIMP3.RSC advances · 2019Article
- TGFβ-induced cytoskeletal remodeling mediates elevation of cell stiffness and invasiveness in NSCLC.Scientific reports · 2019Article
- PI3K inhibitors protect against glucocorticoid-induced skin atrophy.EBioMedicine · 2019Article
- Reactive Oxygen Species and NOX Enzymes Are Emerging as Key Players in Cutaneous Wound Repair.International journal of molecular sciences · 2017Review
- IKKα regulates human keratinocyte migration through surveillance of the redox environment.Journal of cell science · 2017Article
- Oxidation of heat shock protein 60 and protein disulfide isomerase activates ERK and migration of human hepatocellular carcinoma HepG2.Oncotarget · 2016Article
- Dysregulation of Lysyl Oxidase Expression in Lesions and Endometrium of Women With Endometriosis.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
- Xanthine Oxidoreductase Function Contributes to Normal Wound Healing.Molecular medicine (Cambridge, Mass.) · 2015Article
- bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1- JNK Pathways.International journal of biological sciences · 2015Article
- NADPH oxidase NOX4 supports renal tumorigenesis by promoting the expression and nuclear accumulation of HIF2α.Cancer research · 2014Article
- Wild-type and mutant p53 differentially regulate NADPH oxidase 4 in TGF-β-mediated migration of human lung and breast epithelial cells.British journal of cancer · 2014Article
- NADPH oxidase 1 and its derived reactive oxygen species mediated tissue injury and repair.Oxidative medicine and cellular longevity · 2014Review
- Hepatocyte growth factor-loaded biomaterials for mesenchymal stem cell recruitment.Stem cells international · 2013Article
- Nox4 involvement in TGF-beta and SMAD3-driven induction of the epithelial-to-mesenchymal transition and migration of breast epithelial cells.Free radical biology & medicine · 2012Article
- Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation.PloS one · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wound healing requires re-epithelialization from the wound margin through keratinocyte proliferation and migration, and some growth factors are known to influence this process. In the present study, we found that the co-treatment with hepatocyte growth factor (HGF) and TGF-beta1 resulted in enhanced migration of HaCaT cells compared with either growth factor alone, and that N-acetylcysteine, an antioxidant agent, was the most effective among several inhibitors tested, suggesting the involvement of reactive oxygen species (ROS). Fluorescence-activated cell sorter analysis using 2,7-dichlorofluorescein diacetate (DCF-DA) dye showed an early (30 min) as well as a late (24 h) increase of ROS after scratch, and the increase was more prominent with the growth factor treatment. Diphenyliodonium (DPI), a potent inhibitor of NADPH oxidase, abolished the increase of ROS at 30 min, followed by the inhibition of migration, but not the late time event. More precisely, gene knockdown by shRNA for either Nox-1 or Nox-4 isozyme of gp91phox subunit of NADPH oxidase abolished both the early time ROS production and migration. However, HaCaT cell migration was not enhanced by treatment with H((2))O((2)). Collectively, co-treatment with HGF and TGF-beta1 enhances keratinocyte migration, accompanied with ROS generation through NADPH oxidase, involving Nox-1 and Nox-4 isozymes.
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