ArticleProceedings of the National Academy of Sciences of the United States of America2010
High-throughput identification of protein localization dependency networks.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- Organizing organelles: bacterial strategies for localizing intracellular compartments.Current opinion in microbiology · 2025Review
- Large-scale identification of genes involved in septal pore plugging in multicellular fungi.Nature communications · 2023Article
- Experimental and Analytical Approaches for Improving the Resolution of Randomly Barcoded Transposon Insertion Sequencing (RB-TnSeq) Studies.ACS synthetic biology · 2022Article
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
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- The type IV pilin PilA couples surface attachment and cell-cycle initiation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Discovery and Therapeutic Targeting of Differentiated Biofilm Subpopulations.Frontiers in microbiology · 2019Review
- MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis.Nature microbiology · 2016Article
- New Technologies for Studying Biofilms.Microbiology spectrum · 2015Review
- Dynamical Localization of DivL and PleC in the Asymmetric Division Cycle of Caulobacter crescentus: A Theoretical Investigation of Alternative Models.PLoS computational biology · 2015Article
- Effects of (p)ppGpp on the progression of the cell cycle of Caulobacter crescentus.Journal of bacteriology · 2014Article
- Increasing the Content of High-Content Screening: An Overview.Journal of biomolecular screening · 2014Review
- Cell sorting enriches Escherichia coli mutants that rely on peptidoglycan endopeptidases to suppress highly aberrant morphologies.Journal of bacteriology · 2013Article
- Analysis of gene expression levels in individual bacterial cells without image segmentation.Biochemical and biophysical research communications · 2012Article
- Image analysis in fluorescence microscopy: bacterial dynamics as a case study.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Review
- Structure of the pilus assembly protein TadZ from Eubacterium rectale: implications for polar localization.Molecular microbiology · 2012Article
- An SMC ATPase mutant disrupts chromosome segregation in Caulobacter.Molecular microbiology · 2011Article
- Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions.PLoS genetics · 2011Article
- The three-dimensional architecture of a bacterial genome and its alteration by genetic perturbation.Molecular cell · 2011Article
- The essential genome of a bacterium.Molecular systems biology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial cells are highly organized with many protein complexes and DNA loci dynamically positioned to distinct subcellular sites over the course of a cell cycle. Such dynamic protein localization is essential for polar organelle development, establishment of asymmetry, and chromosome replication during the Caulobacter crescentus cell cycle. We used a fluorescence microscopy screen optimized for high-throughput to find strains with anomalous temporal or spatial protein localization patterns in transposon-generated mutant libraries. Automated image acquisition and analysis allowed us to identify genes that affect the localization of two polar cell cycle histidine kinases, PleC and DivJ, and the pole-specific pili protein CpaE, each tagged with a different fluorescent marker in a single strain. Four metrics characterizing the observed localization patterns of each of the three labeled proteins were extracted for hundreds of cell images from each of 854 mapped mutant strains. Using cluster analysis of the resulting set of 12-element vectors for each of these strains, we identified 52 strains with mutations that affected the localization pattern of the three tagged proteins. This information, combined with quantitative localization data from epitasis experiments, also identified all previously known proteins affecting such localization. These studies provide insights into factors affecting the PleC/DivJ localization network and into regulatory links between the localization of the pili assembly protein CpaE and the kinase localization pathway. Our high-throughput screening methodology can be adapted readily to any sequenced bacterial species, opening the potential for databases of localization regulatory networks across species, and investigation of localization network phylogenies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.