ArticleThe Journal of cell biology2010
Directly probing the mechanical properties of the spindle and its matrix.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Microtubule regulation in cancer cells.Frontiers in cell and developmental biology · 2025Article
- Modulation of the cytoskeleton for cancer therapy.Frontiers in cell and developmental biology · 2025Article
- Article
- Opposing motors provide mechanical and functional robustness in the human spindle.Developmental cell · 2021Article
- Individual kinetochore-fibers locally dissipate force to maintain robust mammalian spindle structure.The Journal of cell biology · 2020Article
- Active forces shape the metaphase spindle through a mechanical instability.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- The Spindle: Integrating Architecture and Mechanics across Scales.Trends in cell biology · 2018Review
- High-quality frozen extracts ofMolecular biology of the cell · 2017Article
- Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy.Current biology : CB · 2017Article
- Metaphase Spindle Assembly.Biology · 2017Review
- Mechanical properties of spindle poles are symmetrically balanced.Biophysics and physicobiology · 2017Article
- Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore.The Journal of cell biology · 2016Article
- Active contraction of microtubule networks.eLife · 2015Article
- Article
- Article
- Modelling chromosome dynamics in mitosis: a historical perspective on models of metaphase and anaphase in eukaryotic cells.Interface focus · 2014Review
- A computational model for the formation of lamin-B mitotic spindle envelope and matrix.Interface focus · 2014Article
- Micromechanics of the vertebrate meiotic spindle examined by stretching along the pole-to-pole axis.Biophysical journal · 2014Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Several recent models for spindle length regulation propose an elastic pole to pole spindle matrix that is sufficiently strong to bear or antagonize forces generated by microtubules and microtubule motors. We tested this hypothesis using microneedles to skewer metaphase spindles in Xenopus laevis egg extracts. Microneedle tips inserted into a spindle just outside the metaphase plate resulted in spindle movement along the interpolar axis at a velocity slightly slower than microtubule poleward flux, bringing the nearest pole toward the needle. Spindle velocity decreased near the pole, which often split apart slowly, eventually letting the spindle move completely off the needle. When two needles were inserted on either side of the metaphase plate and rapidly moved apart, there was minimal spindle deformation until they reached the poles. In contrast, needle separation in the equatorial direction rapidly increased spindle width as constant length spindle fibers pulled the poles together. These observations indicate that an isotropic spindle matrix does not make a significant mechanical contribution to metaphase spindle length determination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.