ArticleThe Journal of general physiology2010
Nitric oxide induces airway smooth muscle cell relaxation by decreasing the frequency of agonist-induced Ca2+ oscillations.
Article in The Journal of general physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 82 citations in OpenAlex.
- Phosphodiesterase V inhibition reduces airway responsiveness, but does not improve the beneficial effect of deep inspiration.Respiration; international review of thoracic diseases · 2013Trial
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- A Unified Map of Airway Interactions: Secretome and Mechanotransduction Loops from Development to Disease.Advances in respiratory medicine · 2025Review
- Sensing molecular carbon dioxide: a translational focus for respiratory disease.Physiological reviews · 2025Review
- IL-28A/IL-10Rβ axis promotes angiogenesis via eNOS/AKT signaling and AP-1/NF-κB/MMP-2 network by regulating HSP70-1 expression.Journal of advanced research · 2025Article
- Therapeutic Potential of Arginine-Loaded Red Blood Cell Nanovesicles Targeting Obese Asthma.Mediators of inflammation · 2025Article
- Reassessing the role of nitric oxide in the pathogenesis of sphincter of Oddi dysfunction.Gastroenterology report · 2025Review
- High throughput screening of airway constriction in mouse lung slices.Scientific reports · 2024Article
- Calcium Signaling in Airway Epithelial Cells: Current Understanding and Implications for Inflammatory Airway Disease.Arteriosclerosis, thrombosis, and vascular biology · 2024Review
- Melatonin mediates phenolic acids accumulation in barley sprouts under MeJA stress.Frontiers in nutrition · 2024Article
- Metallothionein Cup1 attenuates nitrosative stress in the yeastMicrobial cell (Graz, Austria) · 2023Article
- Perspectives on precision cut lung slices-powerful tools for investigation of mechanisms and therapeutic targets in lung diseases.Frontiers in pharmacology · 2023Review
- The differential roles of the two NO-GC isoforms in adjusting airway reactivity.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle.Journal of visualized experiments : JoVE · 2022Article
- Skin Microhemodynamics and Mechanisms of Its Regulation in Type 2 Diabetes Mellitus.Biophysics · 2022Article
- The bitter end: T2R bitter receptor agonists elevate nuclear calcium and induce apoptosis in non-ciliated airway epithelial cells.Cell calcium · 2022Article
- Ginger metabolites and metabolite-inspired synthetic products modulate intracellular calcium and relax airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Bile acids inhibit cholinergic constriction in proximal and peripheral airways from humans and rodents.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- A New Pathway to Airway Relaxation: Targeting the "Other" Cyclase in Asthma.American journal of respiratory cell and molecular biology · 2020Article
- Soluble Guanylate Cyclase Agonists Induce Bronchodilation in Human Small Airways.American journal of respiratory cell and molecular biology · 2020Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Nitric oxide (NO) induces airway smooth muscle cell (SMC) relaxation, but the underlying mechanism is not well understood. Consequently, we investigated the effects of NO on airway SMC contraction, Ca(2+) signaling, and Ca(2+) sensitivity in mouse lung slices with phase-contrast and confocal microscopy. Airways that were contracted in response to the agonist 5-hydroxytryptamine (5-HT) transiently relaxed in response to the NO donor, NOC-5. This NO-induced relaxation was enhanced by zaprinast or vardenafil, two selective inhibitors of cGMP-specific phosphodiesterase-5, but blocked by ODQ, an inhibitor of soluble guanylyl cyclase, and by Rp-8-pCPT-cGMPS, an inhibitor of protein kinase G (PKG). Simultaneous measurements of airway caliber and SMC [Ca(2+)](i) revealed that airway contraction induced by 5-HT correlated with the occurrence of Ca(2+) oscillations in the airway SMCs. Airway relaxation induced by NOC-5 was accompanied by a decrease in the frequency of these Ca(2+) oscillations. The cGMP analogues and selective PKG activators 8Br-cGMP and 8pCPT-cGMP also induced airway relaxation and decreased the frequency of the Ca(2+) oscillations. NOC-5 inhibited the increase of [Ca(2+)](i) and contraction induced by the photolytic release of inositol 1,4,5-trisphosphate (IP(3)) in airway SMCs. The effect of NO on the Ca(2+) sensitivity of the airway SMCs was examined in lung slices permeabilized to Ca(2+) by treatment with caffeine and ryanodine. Neither NOC-5 nor 8pCPT-cGMP induced relaxation in agonist-contracted Ca(2+)-permeabilized airways. Consequently, we conclude that NO, acting via the cGMP-PKG pathway, induced airway SMC relaxation by predominately inhibiting the release of Ca(2+) via the IP(3) receptor to decrease the frequency of agonist-induced Ca(2+) oscillations.
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